Nanofibers Comprising Polystyrene and Zinc Acetate for Extraction of Salivary Histidine Prior to Its Determination by HPLC-DAD

• Published in: Analytical Sciences Vol. 38; no. 1; pp. 21P134 - 112
• Main Authors: WU, Xiaoxiao, HUANG, Jingjing, ZHANG, Chi, KANG, Xuejun
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2022; Springer Singapore
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Original Paper; Nanofibers comprising polystyrene and zinc acetate; Salivary histidine; Extraction; Determination; HPLC–DAD
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.21P134

A novel nanofiber-based solid phase extraction (NFSPE) coupled with HPLC–DAD was developed for the determination of salivary histidine, a new potential index of physiological events. The treatment of the sample was based on a coupling reaction of histidine with the diazotization products of sulfanilic acid and sodium nitrite in a slightly alkaline medium, followed by NFSPE. Nanofibers comprising polystyrene and zinc acetate were used as a novel solid-phase sorbent to extract derived histidine via the chelation of Zn 2+  with the imidazole group in the analyte. The eluted target compound was analyzed by HPLC–DAD. Good linearity was achieved with the concentrations of histidine ranging from 0.645 to 64.5 µM; the detection limit was 0.193 µM. The intra-day and inter-day coefficients of variation were less than 8.0%. The method was successfully applied to the determination of salivary histidine in the subjects exposed to a public speaking stress. Graphical abstract