Nanofibers Comprising Polystyrene and Zinc Acetate for Extraction of Salivary Histidine Prior to Its Determination by HPLC-DAD
A novel nanofiber-based solid phase extraction (NFSPE) coupled with HPLC–DAD was developed for the determination of salivary histidine, a new potential index of physiological events. The treatment of the sample was based on a coupling reaction of histidine with the diazotization products of sulfanil...
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Published in | Analytical Sciences Vol. 38; no. 1; pp. 21P134 - 112 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Singapore
The Japan Society for Analytical Chemistry
2022
Springer Singapore |
Subjects | |
Online Access | Get full text |
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Summary: | A novel nanofiber-based solid phase extraction (NFSPE) coupled with HPLC–DAD was developed for the determination of salivary histidine, a new potential index of physiological events. The treatment of the sample was based on a coupling reaction of histidine with the diazotization products of sulfanilic acid and sodium nitrite in a slightly alkaline medium, followed by NFSPE. Nanofibers comprising polystyrene and zinc acetate were used as a novel solid-phase sorbent to extract derived histidine via the chelation of Zn
2+
with the imidazole group in the analyte. The eluted target compound was analyzed by HPLC–DAD. Good linearity was achieved with the concentrations of histidine ranging from 0.645 to 64.5 µM; the detection limit was 0.193 µM. The intra-day and inter-day coefficients of variation were less than 8.0%. The method was successfully applied to the determination of salivary histidine in the subjects exposed to a public speaking stress.
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0910-6340 1348-2246 |
DOI: | 10.2116/analsci.21P134 |