Transmembrane protein 85 from both human (TMEM85) and yeast (YGL231c) inhibit hydrogen peroxide mediated cell death in yeast

Anti-apoptotic proteins are involved in modulating the process of apoptosis. Here, we report the identification of the previously uncharacterized transmembrane domain protein 85 (TMEM85) as a novel anti-apoptotic sequence. Using growth and viability assays, we demonstrate that the heterologous expre...

Full description

Saved in:
Bibliographic Details
Published inFEBS letters Vol. 582; no. 17; pp. 2637 - 2642
Main Authors Ring, Giselle, Khoury, Chamel M., Solar, Aidan J., Yang, Zhao, Mandato, Craig A., Greenwood, Michael T.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 23.07.2008
Subjects
Alternative Splicing
Amino Acid Sequence
amino acid(s)
Anti-apoptosis
Apoptosis
cDNA
dithiothreitol
DNA complimentary to mRNA
DTT
Gene structure
Genome, Human
H2O2
Humans
hydrogen peroxide
Hydrogen Peroxide - metabolism
Hydrogen Peroxide - pharmacology
Membrane Proteins - genetics
Membrane Proteins - metabolism
Molecular Sequence Data
Oxidative Stress
reverse transcriptase-polymerase chain reaction
RT-PCR
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
TMEM85
transmembrane domain protein 85
Yeast
yeast nitrogen base
YNB
aa
Yeast
DTT
RT-PCR
Gene structure
cDNA
TMEM85
Anti-apoptosis
H2O2
YNB
Apoptosis
Online AccessGet full text

Cover

More Information
Summary:Anti-apoptotic proteins are involved in modulating the process of apoptosis. Here, we report the identification of the previously uncharacterized transmembrane domain protein 85 (TMEM85) as a novel anti-apoptotic sequence. Using growth and viability assays, we demonstrate that the heterologous expression of human TMEM85 in yeast promotes growth and prevents cell death in response to oxidative stress. Overexpression of the yeast TMEM85 ortholog (YGL231c) also leads to increased resistance to oxidative stress. Analysis of the existing TMEM85 DNA complimentary to mRNAs revealed that the human TMEM85 gene is alternatively spliced to produce multiple transcripts and proteins. Thus TMEM85 is a complex gene that encodes a novel conserved anti-apoptotic protein.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2008.06.042