Shotgun collision-induced dissociation of peptides using a time of flight mass analyzer

• Published in: Proteomics (Weinheim) Vol. 3; no. 6; pp. 847 - 850
• Main Authors: Purvine, Samuel, Eppel, Jason-Thomas, Yi, Eugene C., Goodlett, David R.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.06.2003; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Feasibility Studies; Fundamental and applied biological sciences. Psychology; General aspects, investigation methods; In-source collision-induced dissociation; Mass Spectrometry - methods; Parallel peptide tandem mass spectrometry; Peptide Fragments - chemistry; Peptide mixtures; Peptides - chemistry; Proteins; Saccharomyces cerevisiae Proteins - isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Serial; Peptides; Time of flight spectrometer; Collision; Proteomics; Dissociation; Mass spectrometry; Result
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200300362

Parallel collision‐induced dissociation (CID) of peptides rather than serial, as is customary, results in loss of the obvious parent‐fragment ion lineage available from CID on a single ion. We report proof‐of‐principle results suggesting the feasibility of parallel peptide CID, referred to here as shotgun CID, for protein identification when using the measured mass accuracies available from a time of flight mass analyzer and currently available search routines such as SEQUEST. Additionally, we report that parent‐fragment ion lineage may be reconstructed from information encoded in the chromatographic single ion current traces of peptides.