Single‐cell RNA sequencing reveals cellular and molecular reprograming landscape of gliomas and lung cancer brain metastases

• Published in: Clinical and translational medicine Vol. 12; no. 11; pp. e1101 - n/a
• Main Authors: Sun, He‐Fen, Li, Liang‐Dong, Lao, I‐Weng, Li, Xuan, Xu, Bao‐Jin, Cao, Yi‐Qun, Jin, Wei
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.11.2022; John Wiley and Sons Inc; Wiley
• Subjects: Brain cancer; brain metastasis; Brain Neoplasms - genetics; Brain Neoplasms - pathology; CD8-Positive T-Lymphocytes - pathology; Cells; Clinical medicine; Communication; Endothelial Cells - pathology; Gene expression; Genomes; Glioma; Glioma - genetics; Humans; Immunotherapy; Kinases; lung adenocarcinoma; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Mann-Whitney U test; Metastasis; Mutation; programmed cell death 1 ligand 1 (PDL1); Reactive oxygen species; Sequence Analysis, RNA; single‐cell RNA sequencing; Standard scores; Survival analysis; Tumor Microenvironment - genetics; Tumors; tumour microenvironment; single-cell RNA sequencing; glioma; brain metastasis; tumour microenvironment; programmed cell death 1 ligand 1 (PDL1); lung adenocarcinoma
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1002/ctm2.1101

Background Brain malignancies encompass gliomas and brain metastases originating from extracranial tumours including lung cancer. Approximately 50% of patients with lung adenocarcinoma (LUAD) will eventually develop brain metastases. However, the specific characteristics of gliomas and lung‐to‐brain metastases (LC) are largely unknown. Methods We applied single‐cell RNA sequencing to profile immune and nonimmune cells in 4 glioma and 10 LC samples. Results Our analysis revealed that tumour microenvironment (TME) cells are present in heterogeneous subpopulations. LC reprogramed cells into immune suppressed state, including microglia, macrophages, endothelial cells, and CD8+ T cells, with unique cell proportions and gene signatures. Particularly, we identified that a subset of macrophages was associated with poor prognosis. ROS (reactive oxygen species)‐producing neutrophils was found to participant in angiogenesis. Furthermore, endothelial cells participated in active communication with fibroblasts. Metastatic epithelial cells exhibited high heterogeneity in chromosomal instability (CIN) and cell population. Conclusions Our findings provide a comprehensive understanding of the heterogenicity of the tumor microenvironment and tumour cells and it will be crucial for successful immunotherapy development for brain metastasis of lung cancer. Single‐cell transcriptional profiles in gliomas and lung‐to‐brain metastases. TAMs (macrophage and microglia) exhibited phenotypic and functional diversity. Endothelial cells communicated with neutrophils or fibroblasts to support angiogenesis. Metastatic epithelial cells exhibited higher chromosomal instability and enriched a subpopulation with stem cell‐like phenotype.