Analysis of a ribose transport operon from Bacillus subtilis

• Published in: Microbiology (Society for General Microbiology) Vol. 140; no. 8; pp. 1829 - 1838
• Main Authors: Woodson, Karen, Devine, Kevin M
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.1994; Society for General Microbiology
• Subjects: Amino Acid Sequence; Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Proteins - physiology; Bacteriology; Base Sequence; Biological and medical sciences; Biological Transport - genetics; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cloning, Molecular; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetics; Microbiology; Molecular Sequence Data; Operon; Promoter Regions, Genetic; Ribose - metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Transcription Factors - physiology; Nucleotide sequence; Transcription; Bacillus subtilis; Operon; Bacillaceae; Gene expression; Regulation(control); Bacillales; Ribose; Membrane transport; Bacteria; Aminoacid sequence; Catabolic repression
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-140-8-1829

1 National Pharmaceutical Biotechnology Centre, Trinity College, Dublin 2, Ireland 2 Department of Genetics, Trinity College, Dublin 2, Ireland ABSTRACT The csa-15 locus of Bacillus subtilis corresponds to an operon encoding proteins which display features characteristic of the ABC group of transporters. Sequence analysis reveals a very high level of identity to the ribose transport operon of Escherichia coli. This hypothesis is supported by the observation that strains carrying mutagenic insertions in this operon are unable to grow on ribose as sole carbon source. Expression of this operon is directed by a single SigA-type promoter which is negatively regulated by Spo0A during the late-exponential/transition state of the growth cycle. Expression is also subject to catabolite repression and this mode of regulation is dominant to control of expression by Spo0A. Author for correspondence: Kevin M. Devine. Tel: +353 1 7021872. Fax: +353 1 6798558. e-mail: KDEVINE@VAXI.TCD.IE Keywords: Bacillus subtilis , ribose transport operon, Spo0A, catabolite repression Present address: Department of Laboratory Medicine, Washington University Medical School, 660 S. Euclid, St Louis, MO 63110, USA. The GenBank accession number for the sequence reported in this paper is 225798.