The protective effects of green tea extract against L-arginine toxicity to cultured human mesangial cells

• Published in: Journal of Korean medical science Vol. 24 Suppl; no. Suppl 1; pp. S204 - S209
• Main Authors: Shin, Byung Chul, Ryu, Hyun Ho, Chung, Jong Hoon, Lee, Byoung Rai, Kim, Hyun Lee
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Academy of Medical Sciences 01.01.2009; 대한의학회
• Subjects: Antioxidants - metabolism; Arginine - metabolism; Arginine - pharmacology; Arginine - toxicity; Cell Line; Cell Proliferation; Cell Survival; Flavonoids - metabolism; Glomerular Mesangium - cytology; Glomerular Mesangium - metabolism; Humans; Mesangial Cells - cytology; Mesangial Cells - metabolism; Nitric Oxide - chemistry; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - metabolism; Original; Phenols - metabolism; Polyphenols; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Tea; 의학일반; Polyphenols; Green Tea Extract; Nitric Oxide; Arginine
• ISSN: 1011-8934; 1598-6357
• DOI: 10.3346/jkms.2009.24.s1.s204

The aim of this study was to investigate whether green tea extract (GTE) has the protective effects on excess L-arginine induced toxicity in human mesangial cell. Human mesangial cells treated with L-arginine were cultured on Dulbecco's modified eagle medium in the presence and absence of inducible nitric oxide synthase (iNOS) inhibitor and GTE. The cell proliferation was determined by 3 (4,5-dimethylthiazol-2-yl)-2, 5-diphengltetrqzolium bromide, a tetrazole assay. The iNOS mRNA and its protein expression were detected by reverse transcription polymerase chain reaction and Western blot, respectively. The concentration of nitric oxide (NO) was measured by NO enzyme-linced immuno sorbent assay kit. L-arginine significantly inhibited the proliferation of human mesangial cells, and induced the secretion of NO to the media. NO production by L-arginine was significantly suppressed by GTE and iNOS inhibitor (p<0.01). The expression level of iNOS mRNA and its protein that was significantly increased by L-arginine was decreased by iNOS inhibitor but not by GTE. GTE protected the mesangial cells from the NO-mediated cytotoxicity by scavenging the NO rather than by iNOS gene expression. Therefore, we conclude that GTE has some protective effect for renal cells against oxidative injury possibly by polyphenols contained in GTE.