CD73/ecto-5'-nucleotidase protects against vascular inflammation and neointima formation

• Published in: Circulation (New York, N.Y.) Vol. 113; no. 17; pp. 2120 - 2127
• Main Authors: ZERNECKE, Alma, BIDZHEKOV, Kiril, ÖZÜYAMAN, Burcin, FRAEMOHS, Line, LIEHN, Elisa A, LÜSCHER-FIRZLAFF, Juliane M, LTISCHER, Bernhard, SCHRADER, Jurgen, WEBER, Christian
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 02.05.2006
• Subjects: 5'-Nucleotidase - physiology; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Atherosclerosis (general aspects, experimental research); Atherosclerosis - etiology; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Emergency and intensive care: renal failure. Dialysis management; Hyperplasia; Integrin alpha4beta1 - physiology; Intensive care medicine; Male; Medical sciences; Mice; Monocytes - physiology; NF-kappa B - physiology; Tunica Intima - pathology; Vascular Cell Adhesion Molecule-1 - genetics; Vascular Cell Adhesion Molecule-1 - physiology; Vasculitis - prevention & control; Nucleotidase; cell adhesion molecules; Enzyme; Leukocyte; Cell adhesion molecule; Phosphoric monoester hydrolases; Cardiovascular disease; Esterases; Inflammation; Endothelium; Hydrolases; leukocytes; lesion
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/circulationaha.105.595249

Although CD73/ecto-5'-nucleotidase has been implicated in maintaining vasoprotection, its role in regulating endothelial adhesion molecule or inflammatory monocyte recruitment (eg, in the context of vascular injury) remains to be defined. Compared with wild-type mice, CD73-deficient (CD73(-/-)) mice exhibit increased luminal staining and protein and transcript expression for vascular cell adhesion molecule (VCAM)-1 in carotid arteries. In vitro, aortic endothelial cells (ECs) from CD73(-/-) mice display an upregulation of mRNA and protein expression of VCAM-1, associated with increased nuclear factor (NF)-kappaB activity, as determined by chromatin cross-linking and immunoprecipitation or quantitative p65 binding assays. CD73(-/-) ECs and carotid arteries perfused ex vivo supported increased monocyte arrest under flow conditions, which was mediated by alpha(4beta1) integrin. After wire injury of carotid arteries, CD73 expression and activity were upregulated in wild-type mice, whereas neointimal plaque formation and macrophage content were increased in CD73(-/-) mice versus wild-type mice, concomitant with elevated NF-kappaB activation, luminal VCAM-1 expression, and soluble VCAM-1 concentrations. In contrast, reconstitution of wild-type mice with CD73(-/-) versus CD73(+/+) BM did not significantly exacerbate neointima formation. Treatment with the specific A2A receptor agonist ATL-146e reversed the increased VCAM-1 transcript and protein expression in CD73(-/-) ECs and inhibited monocyte arrest on CD73(-/-) ECs. Continuous infusion of ATL-146e prevented neointima formation in CD73(-/-) mice. Our data epitomize the importance of vascular CD73 in limiting endothelial activation and monocyte recruitment via generation of adenosine acting through the A2A receptor, providing a molecular basis for therapeutic protection against vascular inflammation and neointimal hyperplasia.