Hyperspectral imaging for monitoring oxygen saturation levels during normothermic kidney perfusion

The development of improved preservation techniques and the reliable assessment of donor grafts are main fields of research in transplantation medicine. Normothermic machine perfusion (NMP) is a promising alternative to static cold storage of organs, maintaining physiological conditions during prese...

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Bibliographic Details
Published inJournal of sensors and sensor systems Vol. 5; no. 2; pp. 313 - 318
Main Authors Tetschke, Florian, Markgraf, Wenke, Gransow, Marian, Koch, Susanne, Thiele, Christine, Kulcke, Axel, Malberg, Hagen
Format Journal Article
LanguageEnglish
Published Gottingen Copernicus GmbH 22.08.2016
Copernicus Publications
Subjects
Algorithms
Cameras
Change detection
Cold storage
Control algorithms
Cysts
Data analysis
Hyperspectral imaging
Image detection
Infrared imagery
Kidneys
Mathematical analysis
Monitoring
Near infrared radiation
Noise
Organs
Oxygen content
Oxygen saturation
Oxygenation
Patient monitoring equipment
Physiological aspects
Physiology
Preservation
Sensors
Surgical apparatus & instruments
Transplantation
United States > US
Massachusetts
Germany
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Summary:The development of improved preservation techniques and the reliable assessment of donor grafts are main fields of research in transplantation medicine. Normothermic machine perfusion (NMP) is a promising alternative to static cold storage of organs, maintaining physiological conditions during preservation. In combination with NMP, we introduce hyperspectral imaging (HSI) as a novel approach for the monitoring of physiological kidney parameters. A line-scan HSI camera system was used to record images of porcine kidneys during NMP. Based on a dual-wavelength algorithm, the oxygen saturation levels were calculated from HSI recordings. Furthermore, we observed HSI images in the near-infrared (NIR) range in order to detect water characteristics of the kidney tissue. We found increasing levels of oxygenation during NMP and could discriminate between perfused and non-perfused areas. Cysts at the renal capsula were characterized by an absorption increase in the NIR band. Within this work, we showed that HSI is able to detect relevant chemical changes during NMP and allows the identification of pathologic variations.
ISSN:2194-878X
2194-8771
2194-878X
DOI:10.5194/jsss-5-313-2016