An evaluation of multiple phenotypic screening methods for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae

• Published in: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Vol. 20; no. 3; pp. 224 - 227
• Main Authors: Hirsch, Elizabeth B, Chang, Kai-Tai, Zucchi, Paola C, Francoeur, Dennis N, Ledesma, Kimberly R, Tam, Vincent H, Lasco, Todd M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.03.2014
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Proteins - biosynthesis; Bacterial Typing Techniques - methods; beta-Lactam Resistance - genetics; beta-Lactamases - biosynthesis; Boronic Acids; Carbapenem; Chromogenic agar; CRE; Hematology, Oncology and Palliative Medicine; Humans; Klebsiella Infections - microbiology; Klebsiella pneumoniae - classification; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - enzymology; Klebsiella pneumoniae - genetics; Microbial Sensitivity Tests; Modified Hodge test; Phenotype; Phenylboronic acid; Sensitivity and Specificity; Phenylboronic acid; Carbapenem; Modified Hodge test; Chromogenic agar; CRE
• ISSN: 1341-321X; 1437-7780
• DOI: 10.1016/j.jiac.2013.10.011

Abstract Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae may display MICs to carbapenems within susceptible or intermediate ranges, prompting confirmatory testing. Four phenotypic methods to detect KPC producers were evaluated against a collection of clinical Enterobacteriaceae isolates. Meropenem–phenylboronic acid double disk synergy testing demonstrated the best performance with 100% sensitivity and specificity.