Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties

• Published in: Marine drugs Vol. 18; no. 8; p. 416
• Main Authors: Ma, Yan, Li, Jie, Zhang, Xin-Yue, Ni, Hao-Dong, Wang, Feng-Biao, Wang, Hai-Ying, Wang, Zhi-Peng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.08.2020; MDPI
• Subjects: Algae; Alginate lyase; Alginates; Alginates - metabolism; Alginic acid; Amino acids; Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Bioinformatics; Catalysis; Enzyme Stability; Enzymes; Extracellular; extracellular enzymes; Food; Food industry; Foods; Genes; Hydrogen-Ion Concentration; Industrial applications; Industry; Intracellular; intracellular enzymes; Ions; Lyases; Metal ion-resisted; Metal ions; Metals; Molecular weight; Oligosaccharides; pH effects; pH-stable; Phaeophyceae; Pharmaceutical industry; Polysaccharide-Lyases - chemistry; Polysaccharide-Lyases - isolation & purification; Polysaccharide-Lyases - metabolism; Processing fishery products; Properties; Recombinant Proteins - metabolism; Recombinants; Salinity tolerance; salt tolerance; Seaweed meal; Seaweed processing; Seaweeds; Sodium chloride; Sodium Chloride - chemistry; Substrate Specificity; Temperature; Vibrio; Vibrio - enzymology; Waterborne diseases; Yarrowia lipolytica; Metal ion-resisted; Alginate lyase; Intracellular; pH-stable; Yarrowia lipolytica
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md18080416

Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.