Electrospinning Live Cells Using Gelatin and Pullulan

Electrospinning is a scaffold production method that utilizes electric force to draw a polymer solution into nanometer-sized fibers. By optimizing the polymer and electrospinning parameters, a scaffold is created with the desired thickness, alignment, and pore size. Traditionally, cells and biologic...

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Bibliographic Details
Published inBioengineering (Basel) Vol. 7; no. 1; p. 21
Main Authors Nosoudi, Nasim, Oommen, Anson Jacob, Stultz, Savannah, Jordan, Micah, Aldabel, Seba, Hohne, Chandra, Mosser, James, Archacki, Bailey, Turner, Alliah, Turner, Paul
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 22.02.2020
MDPI
Subjects
Adipose tissue
Bioengineering
Cell adhesion & migration
cell seeding
Cell size
Collagen
Electric fields
Electrospinning
Extracellular matrix
Gelatin
high voltage
live-cell electrospinning
Polymers
Polyvinyl alcohol
Pore size
Porosity
Production methods
Scaffolds
Smooth muscle
Solvents
Stem cells
tissue engineering
Viability
United States > US
live-cell electrospinning
cell seeding
high voltage
tissue engineering
electrospinning
viability
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Summary:Electrospinning is a scaffold production method that utilizes electric force to draw a polymer solution into nanometer-sized fibers. By optimizing the polymer and electrospinning parameters, a scaffold is created with the desired thickness, alignment, and pore size. Traditionally, cells and biological constitutes are implanted into the matrix of the three-dimensional scaffold following electrospinning. Our design simultaneously introduces cells into the scaffold during the electrospinning process at 8 kV. In this study, we achieved 90% viability of adipose tissue-derived stem cells through electrospinning.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:2306-5354
2306-5354
DOI:10.3390/bioengineering7010021