DNA Methylation Mediated by a MicroRNA Pathway
In plants, the known microRNAs (miRNAs) are produced as ∼21 nucleotide (nt) duplexes from their precursors by Dicer-like 1 (DCL1). They are incorporated into Argonaute 1 (AGO1) protein to regulate target gene expression primarily through mRNA cleavage. We report here the discovery of a class of miRN...
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Published in | Molecular cell Vol. 38; no. 3; pp. 465 - 475 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
14.05.2010
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Subjects | |
Online Access | Get full text |
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Summary: | In plants, the known microRNAs (miRNAs) are produced as ∼21 nucleotide (nt) duplexes from their precursors by Dicer-like 1 (DCL1). They are incorporated into Argonaute 1 (AGO1) protein to regulate target gene expression primarily through mRNA cleavage. We report here the discovery of a class of miRNAs in the model monocot rice (
Oryza sativa). These are 24 nt in length and require another member of the Dicer family, DCL3, for their biogenesis. The 24 nt long miRNAs (lmiRNAs) are loaded into AGO4 clade proteins according to hierarchical rules, depending on the upstream biogenesis machinery and the 5′-terminal nucleotide. We demonstrated that lmiRNAs direct DNA methylation at loci from which they are produced as well as in
trans at their target genes and play roles in gene regulation. Considered together, our findings define a miRNA pathway that mediates DNA methylation.
► There are
DCL3-dependent 24 nt long miRNAs (lmiRNAs) in rice ► LmiRNAs are sorted into AGO4 clade proteins ► MicroRNA sorting depends on the biogenesis machinery and the 5′ end nucleotide ► LmiRNAs direct target DNA methylation and play roles in gene regulation |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1097-2765 1097-4164 |
DOI: | 10.1016/j.molcel.2010.03.008 |