Apoptotic Cell-Derived CD14(+) Microparticles Promote the Phagocytic Activity of Neutrophilic Precursor Cells in the Phagocytosis of Apoptotic Cells

• Published in: Cells (Basel, Switzerland) Vol. 12; no. 15; p. 1983
• Main Authors: Lin, Yu-Chieh, Tsai, Wen-Hui, Chang, Shao-Chi, Hsu, Hui-Chi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2023; MDPI
• Subjects: Antigens; Apoptosis; apoptotic cells; CD14; CD14 antigen; Cell culture; Cell differentiation; Cell research; Cytokines; differentiation syndrome; Flow cytometry; Glycoproteins; granulocytic differentiation; Inflammation; Leukemia; Leukocytes (neutrophilic); Lipopolysaccharides; Microparticles; Monoclonal antibodies; neutrophilic precursor cells; Neutrophils; Pathogens; Phagocytes; Phagocytosis; Physiological aspects; Retinoic acid; TLR4 protein; Toll-like receptors; Tumor necrosis factor-TNF; Taiwan; Minneapolis Minnesota; St Louis Missouri; United States > US; phagocytosis; retinoic acid; granulocytic differentiation; apoptotic cells; differentiation syndrome; neutrophilic precursor cells; microparticles; CD14; resolution
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12151983

Membranous CD14 is crucial in the phagocytic activity of neutrophils. However, the role of CD14(+) microparticles (MPs) derived from apoptotic neutrophils (apo-MP) during the phagocytic process is not clear. All trans-retinoic acid (ATRA) induces acute promyelocytic leukemic NB4 cells along granulocytic differentiation. In this study, we investigated the role of CD14(+)apo-MP in the cell-cell interaction during the phagocytic process of apoptotic cells by viable ATRA-NB4 cells. We firstly demonstrate that CD14 expression and phagocytic activity of NB4 cells were upregulated simultaneously after ATRA treatment in a time-dependent manner, and both were significantly enhanced via concurrent lipopolysaccharide treatment. The phagocytic activity of ATRA-NB4 cells and lipopolysaccharide-treated ATRA-NB4 cells were both significantly attenuated by pre-treating cells with an antibody specific to either CD14 or TLR4. Further flow cytometric analysis demonstrates that apoptotic ATRA-NB4 cells release CD14(+)apo-MP in an idarubicin dosage-dependent manner. Both CD14 expression and the phagocytic activity of viable ATRA-NB4 cells were significantly enhanced after incubation with apo-MP harvested from apoptotic ATRA-NB4 cells, and the apo-MP-enhanced phagocytic activity was significantly attenuated by pre-treating apo-MP with an anti-CD14 antibody before incubation with viable cells. We conclude that CD14(+)apo-MP derived from apoptotic ATRA-NB4 cells promotes the phagocytic activity of viable ATRA-NB4 cells in engulfing apoptotic cells.