Effect of Pyridoxine Deficiency on Ornithine Aminotransferase in Rat Kidney and Liver

• Published in: Journal of Nutritional Science and Vitaminology Vol. 31; no. 6; pp. 553 - 561
• Main Authors: IKEDA, Masuko, OKADA, Mitsuko
• Format: Journal Article
• Language: English
• Published: Japan Center for Academic Publications Japan 1985
• Subjects: Animals; Antigens, Surface - analysis; Female; hepatic and renal OAT; Kidney - enzymology; Kidney - immunology; Kidney - metabolism; Liver - enzymology; Liver - immunology; Liver - metabolism; Male; Membrane Proteins; Ornithine-Oxo-Acid Transaminase - metabolism; Protein Biosynthesis; Proteins - analysis; pyridoxal phosphate; pyridoxine deficiency; Rats; Rats, Inbred Strains; Sex Factors; sexual difference; synthesis of OAT; Transaminases - metabolism; Vitamin B 6 Deficiency - enzymology
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.31.553

The hepatic and renal activities of ornithine aminotrans-ferase (OAT) [L-ornithine 2-oxoacid aminotransferase, EC 2.6.1.13] were determined in male and female rats given diet with or without pyridoxine. OAT activities were measured in the presence of pyridoxal phosphate. Diet without pyridoxine caused a decrease in hepatic OAT activity in males and no change in females and an increase in renal OAT activity in males and a decrease in females. The increased renal OAT activity in males was associated with increased immunochemically recognizable OAT, and was due to increased synthesis of OAT as shown by measurement of [3H]leucine incorporation in vivo. Thus OAT is regulated by nutritional conditions in different ways in liver and kidney.