Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications

Liver disease is one of the leading causes of death worldwide, leading to the death of approximately 2 million people per year. Current therapies include orthotopic liver transplantation, however, donor organ shortage remains a great challenge. In addition, the development of novel therapeutics has...

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Published inBioengineering (Basel) Vol. 7; no. 2; p. 36
Main Authors Cotovio, João P, Fernandes, Tiago G
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 09.04.2020
MDPI
Subjects
Bioengineering
Biomedical materials
Cell culture
Cell therapy
disease modeling
Drug development
hepatic cell lineages
hepatocyte differentiation
Hepatocytes
Homeostasis
human pluripotent stem cells
Liver
Liver cancer
Liver diseases
liver organoids
Liver transplantation
Mammals
Metabolism
non-parenchymal liver cells
Organoids
Pluripotency
Review
Stem cells
Therapeutic applications
Transplantation
hepatic cell lineages
drug screening
liver organoids
non-parenchymal liver cells
disease modeling
hepatocyte differentiation
human pluripotent stem cells
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Summary:Liver disease is one of the leading causes of death worldwide, leading to the death of approximately 2 million people per year. Current therapies include orthotopic liver transplantation, however, donor organ shortage remains a great challenge. In addition, the development of novel therapeutics has been limited due to the lack of in vitro models that mimic in vivo liver physiology. Accordingly, hepatic cell lineages derived from human pluripotent stem cells (hPSCs) represent a promising cell source for liver cell therapy, disease modelling, and drug discovery. Moreover, the development of new culture systems bringing together the multiple liver-specific hepatic cell types triggered the development of hPSC-derived liver organoids. Therefore, these human liver-based platforms hold great potential for clinical applications. In this review, the production of the different hepatic cell lineages from hPSCs, including hepatocytes, as well as the emerging strategies to generate hPSC-derived liver organoids will be assessed, while current biomedical applications will be highlighted.
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ISSN:2306-5354
2306-5354
DOI:10.3390/bioengineering7020036