Non-ureolytic calcium carbonate precipitation by Lysinibacillus sp. YS11 isolated from the rhizosphere of Miscanthus sacchariflorus

• Published in: The journal of microbiology Vol. 55; no. 6; pp. 440 - 447
• Main Authors: Lee, Yun Suk, Kim, Hyun Jung, Park, Woojun
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.06.2017; Springer Nature B.V; 한국미생물학회
• Subjects: Aerobic conditions; Ammonia; Anaerobic conditions; Anoxic conditions; Bacillaceae - growth & development; Bacillaceae - isolation & purification; Bacillaceae - metabolism; Bacteria; Binding energy; biofilm; Biofilms; Biomedical and Life Sciences; Calcium; Calcium carbonate; Calcium Carbonate - metabolism; Calcium carbonates; Carbonate minerals; Carbonates; Cells; Chemical Precipitation; Concrete; Electron microscopy; Emission analysis; Energy dispersive X ray spectroscopy; energy-dispersive X-ray analysis; Environmental engineering; Environmental impact; Environmental monitoring; Extracellular; Field emission microscopy; Fields; Growth; Hypoxia; Life Sciences; Lysinibacillus; Microbial Ecology and Environmental Microbiology; microbial growth; Microbiology; Microscopy, Electron, Scanning; Minerals; Miscanthus sacchariflorus; monitoring; Nitrates; Oxic conditions; Poaceae - microbiology; Rhizosphere; RNA, Ribosomal, 16S - genetics; Scanning electron microscopy; Self healing materials; specific ion electrodes; Spectrometry; Spectrometry, X-Ray Emission; Spores; Urea; Urea - metabolism; X-Ray Diffraction; 생물학; X-ray diffraction; aeration; aerobic MICP; sp. YS11; MICP; urea; Lysinibacillus sp. YS11
• ISSN: 1225-8873; 1976-3794; 1976-3794
• DOI: 10.1007/s12275-017-7086-z

Although microbially induced calcium carbonate precipitation (MICP) through ureolysis has been widely studied in environmental engineering fields, urea utilization might cause environmental problems as a result of ammonia and nitrate production. In this study, many non-ureolytic calcium carbonate-precipitating bacteria that induced an alkaline environment were isolated from the rhizosphere of Miscanthus sacchariflorus near an artificial stream and their ability to precipitate calcium carbonate minerals with the absence of urea was investigated. MICP was observed using a phase-contrast microscope and ion-selective electrode. Only Lysinibacillus sp. YS11 showed MICP in aerobic conditions. Energy dispersive X-ray spectrometry and X-ray diffraction confirmed the presence of calcium carbonate. Field emission scanning electron microscopy analysis indicated the formation of morphologically distinct minerals around cells under these conditions. Monitoring of bacterial growth, pH changes, and Ca 2+ concentrations under aerobic, hypoxia, and anaerobic conditions suggested that strain YS11 could induce alkaline conditions up to a pH of 8.9 and utilize 95% of free Ca 2+ only under aerobic conditions. Unusual Ca 2+ binding and its release from cells were observed under hypoxia conditions. Biofilm and extracellular polymeric substances (EPS) formation were enhanced during MICP. Strain YS11 has resistance at high pH and in high salt concentrations, as well as its spore-forming ability, which supports its potential application for self-healing concrete.