Characterization of Streptomyces piniterrae sp. nov. and Identification of the Putative Gene Cluster Encoding the Biosynthesis of Heliquinomycins

• Published in: Microorganisms (Basel) Vol. 8; no. 4; p. 495
• Main Authors: Zhuang, Xiaoxin, Wang, Zhiyan, Peng, Chenghui, Su, Can, Gao, Congting, Wang, Yongjiang, Huang, Shengxiong, Liu, Chongxi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.03.2020; MDPI
• Subjects: Algorithms; Antibiotics; Biosynthesis; biosynthetic gene cluster; Cardiolipin; Deoxyribonucleic acid; DNA; Enzymes; Fatty acids; Genes; genome analysis; Genomes; heliquinomycins; Low level; Menaquinones; Morphology; New species; Nucleotide sequence; Phosphatidylethanolamine; Phosphatidylinositol; Phospholipids; Phylogenetics; Phylogeny; Physical characteristics; Physiology; Pinus yunnanensis; RecA protein; Rhizosphere; RpoB protein; rRNA 16S; Sequence analysis; Spores; Streptomyces; Streptomycespiniterrae sp. nov; Taxonomy; China; biosynthetic gene cluster; heliquinomycins; Streptomycespiniterrae sp. nov; Pinus yunnanensis; genome analysis
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms8040495

A novel actinomycete producing heliquinomycin and 9'-methoxy-heliquinomycin, designated strain jys28 , was isolated from rhizosphere soil of and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus . It formed spiral chains of spores with spiny surfaces. The menaquinones detected were MK-9(H ), MK-9(H ) and MK-9(H ). The major fatty acids were iso-C , C , C ω7с and anteiso-C . The phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylinositol mannoside. The DNA G + C content of the draft genome sequence, consisting of 8.5 Mbp, was 70.6%. Analysis of the 16S rRNA gene sequence showed that strain jys28 belongs to the genus with the highest sequence similarities to NBRC 13058 (99.2%) and DSM 40002 (99.2%) and phylogenetically clustered with them. Multilocus sequence analysis based on five other house-keeping genes ( D, B, B, A and B and the low level of DNA-DNA relatedness and phenotypic differences allowed the novel isolate to be differentiated from its most closely related strains. Therefore, the strain is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. Furthermore, the putative biosynthetic gene cluster of heliquinomycins was identified and the biosynthetic pathway was discussed. The type strain is jys28 (=CCTCC AA 2018051 =DSM 109823 ).