Molecular resolution imaging by repetitive optical selective exposure

• Published in: Nature methods Vol. 16; no. 11; pp. 1114 - 1118
• Main Authors: Gu, Lusheng, Li, Yuanyuan, Zhang, Shuwen, Xue, Yanhong, Li, Weixing, Li, Dong, Xu, Tao, Ji, Wei
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.11.2019; Nature Publishing Group
• Subjects: 631/1647/245/2225; 631/1647/245/2226; 631/1647/328/2238; 631/57/2265; 631/57/2282; Animals; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Brief Communication; Chlorocebus aethiops; COS Cells; Field of view; Fluorescence; Fluorescence microscopy; Interferometry; Life Sciences; Localization; Localization method; Methods; Microscopy, Fluorescence - methods; Proteomics; Single Molecule Imaging - methods; China
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/s41592-019-0544-2

We introduce an interferometric single-molecule localization method for super-resolution fluorescence microscopy. Fluorescence molecules are located by the intensities of multiple excitation patterns of an interference fringe, providing around a twofold improvement in the localization precision compared with the conventional imaging with the same photon budget. We demonstrate this technique by resolving nanostructures down to 5 nm in size over a large 25 × 25 μm 2 field of view. Repetitive optical selective exposure (ROSE) is an interferometric single-molecule localization microscopy method offering twofold improvement in lateral resolution with the same photon budget compared with conventional approaches.