Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model

• Published in: Basic and clinical neuroscience Vol. 12; no. 4; pp. 523 - 532
• Main Authors: Kadkhodaeian, Hamid Aboutaleb, Salati, Amir, Ansari, Mojtaba, Taghdiri Nooshabadi, Vajihe
• Format: Journal Article
• Language: English
• Published: Iran Negah Scientific Publisher 01.07.2021; Iranian Neuroscience Society; Iran University of Medical Sciences
• Subjects: Age; age-related macular degeneration; Animal models; bone marrow stromal stem cells; CD44 antigen; Epithelium; Femur; Fibroblast growth factor 2; Immunocytochemistry; Macular degeneration; Mesenchyme; Nestin; Neurofilaments; neurosphere cells; Oct-4 protein; Research Paper; Retina; Retinal pigment epithelium; sodium iodate; Stem cells; Age-Related macular degeneration; Neurosphere cells; Bone marrow stromal stem cells; Sodium iodate
• ISSN: 2008-126X; 2228-7442; 2228-7442
• DOI: 10.32598/bcn.2021.12.4.2230.1

Retinal Pigment Epithelium (RPE) layer deterioration is a leading cause of Age-Related Macular Degeneration (AMD), i.e., the most significant reason for irreversible blindness. The present study aimed to track the Neurosphere-Derived (NS) from Bone Marrow Stromal Stem Cells (BMSCs) grafted into the sub-retinal space (destruction of the RPE layer by sodium iodate). RPE degeneration model was performed using the injection of 5% sodium iodate performed in the retro-orbital sinus of Wistar rats. BMSCs were extracted from the examined rat femur and induced into NS, using EGF, bFGF, and B27. BrdU-NS labeled cells were transplanted into the sub-retinal space. For detecting BMSCs and NS markers, immunocytochemistry was performed. Moreover, immunohistochemical was conducted for tracking the transplanted cells in the RPE and sensory retina. The immunocytochemistry of BMSCs cells displayed the expression of mesenchymal stem cells markers (CD90; 99%±1), CD166 (98%±2), CD44 (99%±1). Additionally, the expression of neural lineage markers in NS, such as SOX2, OCT4, Nanog, Nestin, and Neurofilaments (68, 160, 200) revealed the differentiation from BMSCs. Tracking BrdU-NS labeled suggested these aggregations in most layers of the retina. Our study data indicated that BMSCs derived neurosphere had the potential to migrate in injured retinal and integrate into the neurosensory retina. These data can be useful in finding safe cells for replacement therapy in AMD.