PUCHI Regulates Giant Cell Morphology During Root-Knot Nematode Infection in Arabidopsis thaliana
Parasitic root-knot nematodes transform the host’s vascular cells into permanent feeding giant cells (GCs) to withdraw nutrients from the host plants. GCs are multinucleated metabolically active cells with distinctive cell wall structures; however, the genetic regulation of GC formation is largely u...
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Published in | Frontiers in plant science Vol. 12; p. 755610 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
06.10.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Parasitic root-knot nematodes transform the host’s vascular cells into permanent feeding giant cells (GCs) to withdraw nutrients from the host plants. GCs are multinucleated metabolically active cells with distinctive cell wall structures; however, the genetic regulation of GC formation is largely unknown. In this study, the functions of the
Arabidopsis thaliana
transcription factor PUCHI during GC development were investigated.
PUCHI
expression was shown to be induced in early developing galls, suggesting the importance of the
PUCHI
gene in gall formation. Despite the
puchi
mutant not differing significantly from the wild type in nematode invasion and reproduction rates,
puchi
GC cell walls appeared to be thicker and lobate when compared to the wild type, while the cell membrane sometimes formed invaginations. In three-dimensional (3D) reconstructions of
puchi
GCs, they appeared to be more irregularly shaped than those in the wild type, with noticeable cell-surface protrusions and folds. Interestingly, the loss-of-function mutant of
3-KETOACYL-COA SYNTHASE 1
showed GC morphology and cell wall defects similar to those of the
puchi
mutant, suggesting that
PUCHI
may regulate GC development
via
very long chain fatty acid synthesis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Plant Cell Biology, a section of the journal Frontiers in Plant Science Edited by: Kengo Morohashi, Michigan State University, United States Reviewed by: Rosemary White, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia Hirokazu Tanaka, Meiji University, Japan |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.755610 |