Antioxidant and Anti-Inflammatory Properties of Recombinant Bifidobacterium bifidum BGN4 Expressing Antioxidant Enzymes

• Published in: Microorganisms (Basel) Vol. 9; no. 3; p. 595
• Main Authors: Lin, Zhaoyan, Ku, Seockmo, Lim, Taehwan, Park, Sun Young, Park, Myeong Soo, Ji, Geun Eog, O'Brien, Keely, Hwang, Keum Taek
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.03.2021; MDPI
• Subjects: Acids; Anti-inflammatory agents; Antioxidants; Bacteria; Bifidobacterium bifidum; Carbon sources; Catalase; Cell culture; Cytokines; Enzymatic activity; Enzymes; Epithelial cells; Epithelium; Hydrogen peroxide; Inflammation; Inflammatory bowel disease; Interleukin 8; Intestine; Intracellular; Large intestine; Lipopolysaccharides; Macrophages; Oxidative stress; Probiotics; Reactive oxygen species; recombinant bifidobacteria; Superoxide dismutase; superoxide peroxidase; Tumor necrosis factor-TNF; Tumor necrosis factor-α; United States > US; recombinant bifidobacteria; superoxide peroxidase; catalase
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms9030595

BGN4-SK (BGN4-SK), a recombinant strain which was constructed from BGN4 (BGN4) to produce superoxide dismutase (SOD) and catalase, was analyzed to determine its antioxidant and anti-inflammatory properties in vitro. Culture conditions were determined to maximize the SOD and catalase activities of BGN4-SK. The viability, intracellular radical oxygen species (ROS) levels, intracellular antioxidant enzyme activities, and pro-inflammatory cytokine levels were determined to evaluate the antioxidant and anti-inflammatory activities of BGN4-SK in human intestinal epithelial cells (HT-29) and murine macrophage cells (RAW 264.7). Antioxidant enzymes (SOD and catalase) were produced at the highest levels when BGN4-SK was cultured for 24 h in a medium containing 500 μM MnSO and 30 μM hematin, with glucose as the carbon source. The viability and intracellular antioxidant enzyme activities of H O -stimulated HT-29 treated with BGN4-SK were significantly higher ( < 0.05) than those of cells treated with BGN4. The intracellular ROS levels of H O -stimulated HT-29 cells treated with BGN4-SK were significantly lower ( < 0.05) than those of cells treated with BGN4. BGN4-SK more significantly suppressed the production of interleukin (IL)-6 ( < 0.05), tumor necrosis factor-α ( < 0.01), and IL-8 ( < 0.05) in lipopolysaccharide (LPS)-stimulated HT-29 and LPS-stimulated RAW 264.7 cells compared to BGN4. These results suggest that BGN4-SK may have enhanced antioxidant activities against oxidative stress in H O -stimulated HT-29 cells and enhanced anti-inflammatory activities in LPS-stimulated HT-29 and RAW 264.7 cells.