The Manganese Peroxidase Gene Family of Trametes trogii : Gene Identification and Expression Patterns Using Various Metal Ions under Different Culture Conditions

• Published in: Microorganisms (Basel) Vol. 9; no. 12; p. 2595
• Main Authors: Zhang, Yu, Dong, Zhongqi, Luo, Yuan, Yang, En, Xu, Huini, Chagan, Irbis, Yan, Jinping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.12.2021; MDPI
• Subjects: Amino acids; Copper; Enzymes; Extracellular enzymes; Fungi; Gene expression; Gene sequencing; Genomes; Hydrogen peroxide; Introns; Life sciences; Lignin; Lignocellulose; Liquid culture; Manganese; Manganese ions; Manganese peroxidase; manganese peroxidase gene family; Metal ions; Mn2; MnP gene; Peptides; Peroxidase; Phylogenetics; Pollutants; Proteins; Software; static culture; Trametes trogii; White rot; White rot fungi; United States > US; China; Mn2; static culture; white rot fungi; Trametes trogii; manganese peroxidase gene family
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms9122595

Manganese peroxidases (MnPs), gene family members of white-rot fungi, are necessary extracellular enzymes that degrade lignocellulose and xenobiotic aromatic pollutants. However, very little is known about the diversity and expression patterns of the MnP gene family in white-rot fungi, especially in contrast to laccases. Here, the gene and protein sequences of eight unique MnP genes of S0301 were characterized. Based on the characteristics of gene sequence, all TtMnPs here belong to short-type hybrid MnP (type I) with an average protein length of 363 amino acids, 5-6 introns, and the presence of conserved cysteine residues. Furthermore, analysis of MnP activity showed that metal ions (Mn and Cu ) and static liquid culture significantly influenced MnP activity. A maximum MnP activity (>14.0 U/mL) toward 2,6-DMP was observed in static liquid culture after the addition of Mn (1 mM) or Cu (0.2 or 2 mM). Moreover, qPCR analysis showed that Mn obviously upregulated the Group I MnP subfamily ( _09901, 09904, 09903, and 09906), while Cu and H O , along with changing temperatures, mainly induced the Group II MnP subfamily ( _11984, 11971, 11985, and 11983), suggesting diverse functions of fungal MnPs in growth and development, stress response, etc. Our studies here systematically analyzed the gene structure, expression, and regulation of the TtMnP gene family in , one of the important lignocellulose-degrading fungi, and these results extended our understanding of the diversity of the MnP gene family and helped to improve MnP production and appilications of strains and other white-rot fungi.