Cupriavidus necator -Produced Polyhydroxybutyrate/Eudragit FS Hybrid Nanoparticles Mitigates Ulcerative Colitis via Colon-Targeted Delivery of Cyclosporine A

Polyhydroxybutyrate (PHB) has emerged as a novel material for replacing various plastics used in the medical field. However, its application as a drug-delivery carrier for colitis-targeted delivery has not been explored. In this study, we used biosynthesized PHB combined with Eudragit FS (EFS) and c...

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Published inPharmaceutics Vol. 14; no. 12; p. 2811
Main Authors Lee, Juho, Saparbayeva, Aruzhan, Hlaing, Shwe Phyu, Kwak, Dongmin, Kim, Hyunwoo, Kim, Jihyun, Lee, Eun Hee, Yoo, Jin-Wook
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 15.12.2022
MDPI
Subjects
Biocompatibility
Biomass
Biosynthesis
Chloride
colitis-targeted drug delivery
Colon
Inflammatory bowel disease
Morphology
Nanoparticles
NMR
Nuclear magnetic resonance
pH-responsive
PHB biosynthesis
polyhydroxybutyrate
Polyvinyl alcohol
Small intestine
Spectrum analysis
Stomach
sustained release
Trace elements
ulcerative colitis
United States > US
South Korea
Cupriavidus necator
sustained release
pH-responsive
PHB biosynthesis
colitis-targeted drug delivery
polyhydroxybutyrate
ulcerative colitis
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Summary:Polyhydroxybutyrate (PHB) has emerged as a novel material for replacing various plastics used in the medical field. However, its application as a drug-delivery carrier for colitis-targeted delivery has not been explored. In this study, we used biosynthesized PHB combined with Eudragit FS (EFS) and cyclosporine A (CSA) to develop pH-responsive controlled CSA-releasing nanoparticles (CSA-PENPs) for colitis-targeted drug delivery and demonstrated its enhanced therapeutic efficacy in a dextran sulfate sodium (DSS)-induced murine colitis model. PHB was successfully biosynthesized in the bacterium , as demonstrated by H-NMR and FT-IR analyses. CSA-PENPs were fabricated via the oil-in-water emulsion solvent evaporation method. Owing to the potent pH-responsive and sustained drug release properties provided by PHB and EFS, CSA-PENPs could deliver a sufficient amount of CSA to inflamed tissues in the distal colon; in contrast, CSA-loaded EFS nanoparticles displayed premature burst release before reaching the target site. Due to enhanced CSA delivery to colitis tissues, CSA-PENPs exhibited potent anti-inflammatory effects in the DSS-induced murine colitis model. Overall, CSA-PENPs could be a promising drug-delivery system for treating ulcerative colitis.
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These authors contributed equally to this work.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14122811