Identification of BTG2 , an antiproliferative p53-dependent component of the DNA damage cellular response pathway

• Published in: Nature genetics Vol. 14; no. 4; pp. 482 - 486
• Main Authors: Wang, Qing, Magaud, Jean-Pierre, Rouault, Jean-Pierre, Berger, Roland, Guéhenneux, Fabienne, Pain, Bertrand, Berthet, Cyril, Samarut, Christiane, Moyret-Lalle, Caroline, Guillot, Céline, Samarut, Jacques, Shaw, Philip, Rimokh, Ruth, Falette, Nicole, Puisieux, Alain, Ozturk, Mehmet, Savatier, Pierre
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 01.12.1996
• Subjects: 3T3 Cells; Amino Acid Sequence; Animals; Biological and medical sciences; Cell Cycle - genetics; Cell Cycle - physiology; Cell cycle, cell proliferation; Cell Division - physiology; Cell Line; Cell physiology; Chromosome Mapping; Chromosomes, Human, Pair 1; Cloning, Molecular; DNA Damage; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genes, Tumor Suppressor; Humans; Hybrid Cells; Immediate-Early Proteins; Life Sciences; Mice; Molecular and cellular biology; Molecular Sequence Data; Neoplasm Proteins - genetics; Proteins - genetics; Proteins - physiology; Sequence Homology, Amino Acid; Tumor Suppressor Protein p53 - physiology; Tumor Suppressor Proteins; Genetic mapping; Human; Cell proliferation; Targeting; Nucleotide sequence; Induction; Chromosome A1; Homology; Cell differentiation; Gene expression; Complementary DNA; Gene; DNA; Cell cycle; Multigene family; Mutation; Repair; BIOLOGIE MOLECULAIRE
• ISSN: 1061-4036; 1546-1718
• DOI: 10.1038/ng1296-482

Cell cycle regulation is critical for maintenance of genome integrity. A prominent factor that guarantees genomic stability of cells is p53 (ref. 1). The P53 gene encodes a transcription factor that has a role as a tumour suppressor. Identification of p53-target genes should provide greater insight into the molecular mechanisms that mediate the tumour suppressor activities of p53. The rodent Pc3/Tis21 gene was initially described as an immediate early gene induced by tumour promoters and growth factors in PC12 and Swiss 3T3 cells. It is expressed in a variety of cell and tissue types and encodes a remarkably labile protein. Pc3/Tis21 has a strong sequence similarity to the human antiproliferative BTG1 gene cloned from a chromosomal translocation of a B-cell chronic lymphocytic leukaemia. This similarity led us to speculate that BTG1 and the putative human homologue of Pc3/Tis21 (named BTG2) were members of a new family of genes involved in growth control and/or differentiation. This hypothesis was recently strengthened by the identification of a new antiproliferative protein, named TOB, which shares sequence similarity with BTG1 and PC3/TIS21 (ref. 7). Here, we cloned and localized the human BTG2 gene. We show that BTG2 expression is induced through a p53-dependent mechanism and that BTG2 function may be relevant to cell cycle control and cellular response to DNA damage.