Dose-Response Effect of Fluoride Dentifrices on De-/Remineralization of Root Dentine in situ

• Published in: Caries research Vol. 54; no. 5-6; pp. 502 - 508
• Main Authors: Leal, José, Ferreira, Robson, Tabchoury, Cinthia, Lingström, Peter, Vale, Glauber
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.12.2020
• Subjects: Animals; Cariostatic Agents - pharmacology; Cattle; Cross-Over Studies; Dentifrices; Dentifrices - pharmacology; Dentin; Dentistry; Fluoride treatment; Fluorides; Fluorides - pharmacology; Humans; Odontologi; Research Article; Tooth Demineralization - drug therapy; Tooth Demineralization - prevention & control; Tooth Remineralization; Toothpastes; Biofilms; Demineralization; Fluoride; Root dentine; Remineralization
• ISSN: 0008-6568; 1421-976X; 1421-976X
• DOI: 10.1159/000510535

The present study aimed to evaluate the effect of fluoride (F) dentifrice with different F concentrations on root dentine de-/remineralization. Ten healthy volunteers took part in this randomized, double-blinded, cross-over, and split-mouth in situ experimental study. During 4 phases of 7 days, they wore a palatal appliance containing 4 bovine dentine blocks (2 sound and 2 with caries) of 4 × 4 × 2 mm. Treatments were performed with silica-based dentifrices containing 0, 700, 1,300, and 5,000 µg F/g (F as NaF). To provide a cariogenic challenge, a 20% sucrose solution was dripped 3 and 8 times daily on the carious-like and sound blocks, respectively. After each experimental phase, the percentage of surface hardness loss (%SHL) or recovery (%SHR) was calculated and the fluoride concentration in the biofilm was determined. The statistical analysis was performed using ANOVA and the Tukey post hoc test with p at 5%. The relationship between variables was analyzed by linear regression. The results showed a lower %SHL when 5,000 µg F/g dentifrice was used but without a statistically significant difference from the conventional one (1,300 µg F/g). Regarding remineralization and F in biofilms, the high-fluoride dentifrice was expressively superior in mineral replacement on the surface and in the F concentration in the biofilms, respectively, compared to the other 3 products (p < 0.05). Also, a significant linear fit between mineral loss/gain, F in biofilms, and fluoride concentration in the dentifrices could be observed. In conclusion, a dose-response F effect was observed, and the high-fluoride dentifrice was effective in enhancing root dentine remineralization in this short-term in situ study.