Attenuation of bovine herpesvirus type 1 by deletion of its glycoprotein G and tk genes and protection against virulent viral challenge

• Published in: Vaccine Vol. 29; no. 48; pp. 8943 - 8950
• Main Authors: Zhang, Minmin, Fu, Shulin, Deng, Mingliang, Xie, Qian, Xu, Haiyang, Liu, Zhengfei, Hu, Changmin, Chen, Huanchun, Guo, Aizhen
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 08.11.2011; Elsevier
• Subjects: Allergy and Immunology; Animals; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Applied microbiology; Biological and medical sciences; blood serum; Bovine herpesvirus 1; Bovine herpesvirus-1 (BoHV-1); calves; Cattle; Cell Line; dexamethasone; Female; Fundamental and applied biological sciences. Psychology; Gene Deletion; genes; Glycoprotein E (gE); Glycoprotein G (gG); glycoproteins; Herpesvirus; Herpesvirus 1, Bovine - genetics; Herpesvirus 1, Bovine - pathogenicity; homologous recombination; immunization; infectious bovine rhinotracheitis; Infectious bovine rhinotracheitis (IBR); Infectious Bovine Rhinotracheitis - immunology; Infectious Bovine Rhinotracheitis - prevention & control; interferon-beta; interferon-gamma; Interferon-gamma - immunology; Male; Microbiology; Miscellaneous; mutants; neutralization; neutralizing antibodies; Rabbits; thymidine kinase; Thymidine kinase (tk); Thymidine Kinase - genetics; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - immunology; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viral Proteins - genetics; Viral Vaccines - genetics; Viral Vaccines - immunology; Virology; virulence; Virus Shedding; viruses; Bovine herpesvirus-1 (BoHV-1); Glycoprotein G (gG); Vaccines; Infectious bovine rhinotracheitis (IBR); Thymidine kinase (tk); Glycoprotein E (gE); Thymidine kinase; Enzyme; Herpesviridae; Transferases; Alphaherpesvirinae; Glycoprotein; Bovine herpesvirus 1; Zoopathogen; Vaccine; Infection; Virus; Glycoprotein E(gE); Gene; Viral disease; Deletion; Infectious bovine rhinotracheitis; Mutation; Veterinary
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2011.09.050

Highlights ► BoHV-1 gG− / tk− mutant could stimulate cell mediated immunity and humoral immunity response. ► The mutant could not be reactivated by dex injection. ► It could provides a good level of protection.