Aneuploid yeast strains exhibit defects in cell growth and passage through START

• Published in: Molecular biology of the cell Vol. 24; no. 9; pp. 1274 - 1289
• Main Authors: Thorburn, Rebecca R, Gonzalez, Christian, Brar, Gloria A, Christen, Stefan, Carlile, Thomas M, Ingolia, Nicholas T, Sauer, Uwe, Weissman, Jonathan S, Amon, Angelika
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01.05.2013
• Series: A Highlights from
• Subjects: Active Transport, Cell Nucleus; Amino Acids - biosynthesis; Aneuploidy; Chromosomes, Artificial, Yeast - genetics; Cyclin-Dependent Kinases - metabolism; Cyclins - genetics; Cyclins - metabolism; G1 Phase Cell Cycle Checkpoints; Gene Expression Regulation, Fungal; Humans; Intracellular Signaling Peptides and Proteins - metabolism; Metabolome; Protein Biosynthesis; Repressor Proteins - metabolism; Saccharomyces cerevisiae - cytology; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Signal Transduction; Transcription, Genetic
• ISSN: 1059-1524; 1939-4586
• DOI: 10.1091/mbc.e12-07-0520

Aneuploidy, a chromosome content that is not a multiple of the haploid karyotype, is associated with reduced fitness in all organisms analyzed to date. In budding yeast aneuploidy causes cell proliferation defects, with many different aneuploid strains exhibiting a delay in G1, a cell cycle stage governed by extracellular cues, growth rate, and cell cycle events. Here we characterize this G1 delay. We show that 10 of 14 aneuploid yeast strains exhibit a growth defect during G1. Furthermore, 10 of 14 aneuploid strains display a cell cycle entry delay that correlates with the size of the additional chromosome. This cell cycle entry delay is due to a delayed accumulation of G1 cyclins that can be suppressed by supplying cells with high levels of a G1 cyclin. Our results indicate that aneuploidy frequently interferes with the ability of cells to grow and, as with many other cellular stresses, entry into the cell cycle.