Inhibition by Protein Kinase C Inhibitor of Expression of Leukocyte Function‐associated Antigen‐1 Molecules in Rat Hepatoma AH66F Cells

• Published in: Cancer science Vol. 88; no. 3; pp. 267 - 272
• Main Authors: Nomura, Masaaki, Sugiura, Norihiko, Moritani, Shuzo, Miyamoto, Ken‐ichi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.1997; Japanese Cancer Association; John Wiley & Sons, Inc
• Subjects: Alkaloids - pharmacology; Animal tumors. Experimental tumors; Animals; Antigens; Biological and medical sciences; Cell adhesion; Cell Adhesion - drug effects; DNA Primers; Enzyme inhibitors; Enzyme Inhibitors - pharmacology; Experimental digestive system and abdominal tumors; Expression; Flow Cytometry; Hepatoma; Kinases; LFA‐1; Liver Neoplasms, Experimental - pathology; Liver Neoplasms, Experimental - physiopathology; Lymphocyte Function-Associated Antigen-1 - biosynthesis; Lymphocytes; Macromolecular Substances; Medical sciences; Mesentery; Mesentery - cytology; Mesentery - drug effects; Mesentery - physiology; mRNA; Polymerase Chain Reaction; Protein kinase C; Protein Kinase C - antagonists & inhibitors; Protein kinase C inhibitor; Proteins; Rat hepatoma AH66F cell; Rats; Staurosporine - analogs & derivatives; T-Lymphocytes - metabolism; Thymocytes; Tumor Cells, Cultured; Tumors; Protein kinase C; Rat; Enzyme; Ascites tumor; Transferases; Rodentia; Cell adhesion molecule; Hepatic disease; Hepatocellular carcinoma; Malignant tumor; Gene expression; Binding protein; Vertebrata; Mammalia; Integrin; Messenger RNA; Animal; Established cell line; Digestive diseases
• ISSN: 0910-5050; 1347-9032; 1349-7006; 1876-4673
• DOI: 10.1111/j.1349-7006.1997.tb00377.x

To examine the mechanism of inhibition by protein kinase C (PKC) inhibitors of the adhesion of highly malignant hepatoma AH66F cells to the mesentery‐derived mesothelial cell (M‐cell) layer through leukocyte function‐associated antigen‐1 (LFA‐1)/intercellular adhesion molecule‐1, the effects of a PKC inhibitor, NA‐382, on the expression of LFA‐1 molecules in AH66F cells were examined and compared with those in thymocytes from normal rats. NA‐382 inhibited the adhesion of AH66F cells to the M‐cell layer and the expression of LFA‐1 on the membrane of the hepatoma cells after treatment for more than 24 h. It was confirmed that AH66F cells express similar mRNAs for LFA‐1 subunits to those of thymocytes, and their levels were also decreased after treatment with NA‐382. On the other hand, the LFA‐1‐mediated adhesion and the expression of both protein and mRNA for LFA‐1 subunits in thymocytes were not changed by the PKC inhibitor. These results suggest that the expression of LFA‐1 molecules in AH66F cells may be regulated by PKC via quite different mechanisms from those in normal lymphocytes