Imbalance of CD4 + CD45R+ and CD4+ CD29+ T helper cell subsets in patients with atopic diseases

• Published in: Clinical and experimental immunology Vol. 83; no. 1; pp. 25 - 29
• Main Authors: SCHAUER, U., JUNG, T., HEYMANNS, J., RIEGER, C. H. L
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1991; Blackwell
• Subjects: Adolescent; Aging - immunology; Allergic diseases; allergy; Antigens, CD - immunology; Antigens, Differentiation - immunology; asthma; Asthma - immunology; atopic dermatitis; Biological and medical sciences; CD4 Antigens - immunology; Child; Child, Preschool; Dermatitis, Atopic - immunology; Flow Cytometry; General aspects; Histocompatibility Antigens - immunology; Humans; Immunoglobulin E - analysis; Immunopathology; Infant; Integrin beta1; Leukocyte Common Antigens; Leukocyte Count; lymphocyte subsets; Medical sciences; T-Lymphocyte Subsets - immunology; T-Lymphocytes, Helper-Inducer - immunology; Human; Allergy; Immunopathology; Skin disease; Respiratory disease; Helper cell; Cell subpopulation; Asthma; Atopy; Atopic dermatitis; T-Lymphocyte; Immunofluorescence; Child
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.1991.tb05582.x

SUMMARY To evaluate the proportion of helper cell subsets we studied 18 children with atopic dermatitis, 30 patients with asthma. 27 healthy age‐matched controls aged 1 to 17 years and 11 atopic controls without symptoms related to atopy, aged 9–22 years. Lymphocytes were isolated from heparinized peripheral blood and the proportion of CD4+CD29+ and CD4+CD45R+ cells was determined by double‐labelling immunofluorescence. Children with atopic dermatitis yielded a significantly (P<0.0l) higher proportion of CD4+CD45R+ (median 75%) cells compared with normal controls (median 66.6%). whereas the proportion of CD4+CD29+ cells was significantly (P<0.0l) lower in patients with atopic dermatitis (median 204 versus 29.6%). Interestingly, the percentage of CD4+CD45R+ cells shows an age‐dependent decline (r= ‐0.67, P<0.0l) in the control group, which is not found in the patient group.