Angiopoietin-1 Reduces VEGF-Stimulated Leukocyte Adhesion to Endothelial Cells by Reducing ICAM-1, VCAM-1, and E-Selectin Expression

• Published in: Circulation research Vol. 89; no. 6; pp. 477 - 479
• Main Authors: Kim, Injune, Moon, Sang-Ok, Park, Sung Kwang, Chae, Soo Wan, Koh, Gou Young
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD American Heart Association, Inc 14.09.2001; Lippincott
• Subjects: Angiopoietin-1; Biological and medical sciences; Blood vessels and receptors; Blotting, Western; Cell Adhesion - drug effects; Cell Adhesion Molecules - drug effects; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell Line; E-Selectin - drug effects; E-Selectin - genetics; E-Selectin - metabolism; Endothelial Growth Factors - pharmacology; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Humans; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - metabolism; Leukocytes - cytology; Leukocytes - drug effects; Lymphokines - pharmacology; Membrane Glycoproteins - pharmacology; RNA, Messenger - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism; Vascular Cell Adhesion Molecule-1 - drug effects; Vascular Cell Adhesion Molecule-1 - genetics; Vascular Cell Adhesion Molecule-1 - metabolism; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors; Vertebrates: cardiovascular system; Human; Endothelial cell; Vascular cell adhesion molecule 1; Angiogenic factor; Intercellular adhesion molecule 1; Leukocyte; E Selectin; Gene expression; Adhesion; In vitro; Cell line; Vascular endothelium growth factor; Blood vessel; Circulatory system; Umbilical cord
• ISSN: 0009-7330; 1524-4571
• DOI: 10.1161/hh1801.097034

Vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang1) are potent vasculogenic and angiogenic factors that hold promise as a means to produce therapeutic vascularization and angiogenesis. However, VEGF also acts as a proinflammatory cytokine by inducing adhesion molecules that bind leukocytes to endothelial cells, an initial and essential step toward inflammation. In the present study, we used human umbilical vascular endothelial cells (HUVECs) to examine the effect of Ang1 on VEGF-induced expression of three adhesion moleculesintercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Interestingly, Ang1 suppressed VEGF-induced expression of these adhesion molecules. Furthermore, Ang1 reduced VEGF-induced leukocyte adhesion to HUVECs. These results demonstrate that Ang1 counteracts VEGF-induced inflammation by reducing VEGF-induced endothelial adhesiveness.