Comparison of four Mycoplasma pneumoniae IgM-, IgG- and IgA-specific enzyme immunoassays in blood donors and patients

• Published in: Clinical microbiology and infection Vol. 10; no. 12; pp. 1094 - 1098
• Main Authors: Csángó, P.A., Pedersen, J.E., Hess, R.D.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01.12.2004; Blackwell Science Ltd; Blackwell
• Subjects: Antibodies, Bacterial - blood; Bacterial diseases; Biological and medical sciences; Blood Donors; enzyme immunoassays; False Positive Reactions; Human bacterial diseases; Humans; IgA; IgM; Immunoenzyme Techniques - methods; Immunoglobulin A - blood; Immunoglobulin G - blood; Immunoglobulin M - blood; Infectious diseases; Medical sciences; Miscellaneous; Mycoplasma Infections - diagnosis; Mycoplasma Infections - epidemiology; Mycoplasma pneumoniae; Mycoplasma pneumoniae - immunology; Mycoplasma pneumoniae - isolation & purification; Sensitivity and Specificity; Serologic Tests; seroprevalence; Mycoplasma pneumoniae; seroprevalence; IgA; Blood donors; enzyme immunoassays; IgM; Prevalence; IgG; Serology; Epidemiology; Mollicutes; Mycoplasmatales; Bacteria; Diagnosis; Blood donor; Human; Antibody; Respiratory disease; Method; Enzyme immunoassay; Mycoplasmataceae; Infection; Bacteriosis; Mycoplasmal infection; Comparative study
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2004.00993.x

Mycoplasma pneumoniae antibodies were studied in 504 blood donors and 102 patients with infections not caused by M. pneumoniae with the use of enzyme immunoassay kits from ThermoLabsystems (L), Savyon (S), Bio-Rad (B) and Novitec (N). Detection frequencies of M. pneumoniae IgM in blood donors were 14.9% (L), 16.0% (S), 2.8% (B) and 3.8% (N), and in patients were 40.2% (L), 42.2% (S), 9.8% (B) and 16.7% (N). Detection frequencies of M. pneumoniae IgA were 68.5% (L) and 22.8% (S), and in 65 respiratory disease patients were 100% (L) and 53.8% (S). Thus, use of some kits may lead to overdiagnosis of M. pneumoniae infections.