Crystallization of a novel metal-containing cupin from Acidobacterium sp. and preliminary diffraction data analysis

Recombinant AciX9_0562 from Acidobacterium sp. MP5ACTX9 (UniProt ID E8WYN5) containing sequence motifs characteristic of the RmlC‐type cupins superfamily and containing Pfam motif PF07883 has been successfully cloned, expressed and purified. AciX9_0562 crystallized in a number of conditions from the...

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Published inActa crystallographica. Section F, Structural biology and crystallization communications Vol. 68; no. 4; pp. 451 - 454
Main Authors Łyskowski, Andrzej, Steiner, Kerstin, Hajnal, Ivan, Steinkellner, Georg, Schwab, Helmut, Gruber, Karl
Format Journal Article
LanguageEnglish
Published 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.04.2012
Subjects
Acidobacteria - chemistry
Acidobacterium sp. MP5ACTX9
AciX9_0562
Crystallization
Crystallization Communications
Crystallography, X-Ray
Crystals
cupins
Data processing
Diffraction
Pipelines
Proteins
Recombinant
Screens
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Summary:Recombinant AciX9_0562 from Acidobacterium sp. MP5ACTX9 (UniProt ID E8WYN5) containing sequence motifs characteristic of the RmlC‐type cupins superfamily and containing Pfam motif PF07883 has been successfully cloned, expressed and purified. AciX9_0562 crystallized in a number of conditions from the Morpheus protein crystallization screen. The best crystal diffracted to 2.7 Å resolution (space group C2221; unit‐cell parameters a = 125.29, b = 254.63, c = 82.99 Å). Structure solution was facilitated by the automated molecular‐replacement pipeline BALBES. The initial solution was automatically rebuilt using the PHENIX AutoBuild wizard, with final R and Rfree values of 0.23 and 0.26, respectively. The structure is currently undergoing manual refinement.
Bibliography:ark:/67375/WNG-S5Q0MKTG-L
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These authors contributed equally to this work.
ISSN:1744-3091
1744-3091
DOI:10.1107/S1744309112006550