Effect of slurry dilution, structural carbohydrates, and exogenous archaea supply on in vitro anaerobe fermentation and methanogens population of swine slurry
Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze‐dried dairy cattle feces were used (external archaea) as co‐inoculum (Co‐i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as...
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Published in | Environmental progress & sustainable energy Vol. 34; no. 1; pp. 54 - 64 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Blackwell Publishing Ltd
01.01.2015
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Subjects | |
Online Access | Get full text |
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Summary: | Artificial slurry (4% dry matter) was prepared using fresh feces and urine obtained from four pregnant sows fed with commercial diet. Freeze‐dried dairy cattle feces were used (external archaea) as co‐inoculum (Co‐i) and structural carbohydrates (CHO: apple pulp; sugar beet pulp; and wheat straw) as substrates. Bottles were incubated (39 ± 1°C for 56 days), and the gas production was measured (mbar) and converted to the volume. A sample of produced gas was taken and analyzed for methane concentration using a gas chromatography. Bottles were opened at days 0, 25, and 56 to determine total bacteria, total, and hydrogenotrophic methanogens archaea (HMA) concentrations using quantitative polymerase chain reaction and population biodiversity using denaturing gradient gel electrophoresis (DGGE). Incubation time reduced the titers of total bacteria and archaea (P < 0.01) but did not modify HMA population. Doses of Co‐i showed a positive correlation with HMA titers, although interacted with an incubation period (P < 0.001); at 5% Co‐inoculation, total bacteria decreased significantly (0–25 days) but remained steady until day 56 (P > 0.05), whereas at 10% Co inoculation, titers decreased constantly. Most of the archaeal DGGE bands were observed in all samples, suggesting a common microbial population origin but Co‐i supply altered the DGGE structure of archaea populations. © 2014 American Institute of Chemical Engineers Environ Prog, 34: 54–64, 2015 |
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Bibliography: | Ministry of Economy and Competitiveness of Spain and the European Union Regional Development Funds - No. AGL2010-20820 ArticleID:EP11952 ark:/67375/WNG-Z7QWS6T6-B istex:180D3300587F79069291670A0DBC8D86A5628440 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1944-7442 1944-7450 |
DOI: | 10.1002/ep.11952 |