Assessment of lipids in skeletal muscle by LCModel and AMARES

• Published in: Journal of magnetic resonance imaging Vol. 30; no. 5; pp. 1124 - 1129
• Main Authors: Weis, Jan, Johansson, Lars, Ortiz-Nieto, Francisco, Ahlström, Håkan
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.11.2009
• Subjects: Adipose Tissue - pathology; Adult; Algorithms; AMARES; Body Mass Index; Humans; LCModel; Linear Models; Lipids - chemistry; magnetic resonance; magnetic resonance imaging; magnetic resonance spectroscopy; Magnetic Resonance Spectroscopy - methods; Male; Medical Radiophysics; Medicin; Medicine; Medicinsk radiofysik; Middle Aged; Models, Statistical; MR spectroscopy; Muscle, Skeletal - metabolism; Muscles - pathology; muscular lipids; quantification; Reproducibility of Results; Software; VETERINARY MEDICINE; VETERINÄRMEDICIN
• ISSN: 1053-1807; 1522-2586; 1522-2586
• DOI: 10.1002/jmri.21900

Purpose To process single voxel spectra of the human skeletal muscle by using an advanced method for accurate, robust, and efficient spectral fitting (AMARES) and by linear combination of model spectra (LCModel). To determine absolute concentrations of extra‐ (EMCL) and intramyocellular lipids (IMCL). Materials and Methods Single‐voxel proton magnetic resonance spectroscopy (PRESS) was used to obtain the spectra of the calf muscles. Unsuppressed water line was used as a concentration reference. A new prior knowledge for AMARES was proposed to estimate the concentrations of EMCL and IMCL. The prior knowledge was derived from the spectrum of vegetable oil. The results were compared with the values estimated by LCModel. Absolute concentrations of total lipid content in millimoles per kilogram wet weight were used for the comparisons. Results Absolute concentrations of total lipid content in skeletal muscle were estimated by AMARES and LCModel. Very good correlation of the total fat (EMCL + IMCL) and IMCL concentrations was achieved between both data processing approaches. Conclusion Assessment the absolute concentrations of muscular lipids by AMARES and LCModel can be performed with comparable accuracy. J. Magn. Reson. Imaging 2009. © 2009 Wiley‐Liss, Inc.