Fucosterol inhibits adipogenesis through the activation of AMPK and Wnt/β-catenin signaling pathways

• Published in: Food science and biotechnology Vol. 26; no. 2; pp. 489 - 494
• Main Authors: Song, Youngwoo, Oh, Ga Hui, Kim, Mi-Bo, Hwang, Jae-Kwan
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society of Food Science and Technology 01.04.2017; Springer Nature B.V; 한국식품과학회
• Subjects: Acetyl-CoA carboxylase; Adenosine monophosphate; Adipogenesis; Algae; AMP; CCAAT/enhancer-binding protein; Chemistry; Chemistry and Materials Science; Cyclin D1; Dishevelled protein; Food Science; Glycogen; Glycogen synthase kinase 3; Kinases; Lipogenesis; Nutrition; Phosphorylation; Protein kinase; Proteins; Signal transduction; siRNA; Sterol regulatory element-binding protein; Sterols; Transcription factors; Wnt protein; β-Catenin; 식품과학; 3T3-L1 preadipocytes; AMPK; adipogenesis; Wnt/β-catenin; fucosterol
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-017-0067-5

Fucosterol is a sterol constituent primarily derived from brown algae. Recently, the antiadipogenic effect of fucosterol has been reported; however, its molecular mechanism remains to be studied. Fucosterol effectively upregulated the phosphorylations of both adenosine monophosphate (AMP)-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), and downregulated the expression levels of lipogenesis-related factors. Moreover, fucosterol activated the major components of the Wnt/β-catenin signaling pathway, including β-catenin, disheveled 2 (DVL2), and cyclin D1 (CCND1), whereas it inactivated glycogen synthase kinase 3β (p-GSK3β) by stimulating its phosphorylation. In the presence or absence of fucosterol, the adipogenic transcriptional factors [peroxisome proliferator activated-receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and sterol regulatory element binding protein-1c (SREBP-1c)] were upregulated by the inhibition of AMPK by compound C or the knockdown of β-catenin by siRNA. Overall, these data demonstrate that fucosterol prevents adipogenesis by mediating both AMPK- and Wnt/β-catenin-signaling pathways.