Effect of the Bonito Insulin on the Insulin Release from Monolayer Culture of Rat Pancreas

The effect of bonito insulin on insulin release was examined in the monolayer culture of rat pancreatic β-cells. The β-cells were preincubated for 5 to 20hr with or without a small dose (100μU/ml) of bonito insulin in the medium containing 100mg% glucose. And then, they were incubated in 300mg% gluc...

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Bibliographic Details
Published inEndocrinologia Japonica Vol. 25; no. 5; pp. 443 - 446
Main Authors OHGAWARA, HISAKO, TASAKA, YOSHIMASA, KANAZAWA, YASUNORI
Format Journal Article
LanguageEnglish
Published Japan The Japan Endocrine Society 01.01.1978
Subjects
Animals
Cells, Cultured
Fishes
Insulin - metabolism
Insulin - pharmacology
Insulin Secretion
Islets of Langerhans - cytology
Islets of Langerhans - metabolism
Rats
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Summary:The effect of bonito insulin on insulin release was examined in the monolayer culture of rat pancreatic β-cells. The β-cells were preincubated for 5 to 20hr with or without a small dose (100μU/ml) of bonito insulin in the medium containing 100mg% glucose. And then, they were incubated in 300mg% glucose alone or together with bonito insulin for 5hr. There was no significant difference between the IRI release from these n-cells with or without bonito insulin. The concentration of bonito insulin was augmented from 100μU/ml to 500, 1, 000 and 2, 000μU/ml. A significant inhibitory effect on the glucose-induced insulin release was observed only after the preincubation for 20hr with 2, 000μU/ml of bonito insulin.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0013-7219
2185-6370
DOI:10.1507/endocrj1954.25.443