Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical...

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Published inScientific reports Vol. 4; no. 1; p. 7033
Main Authors Benedetti, Lorena, Sogne, Elisa, Rodighiero, Simona, Marchesi, Davide, Milani, Paolo, Francolini, Maura
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 13.11.2014
Nature Publishing Group
Subjects
13
14/19
14/35
14/63
147/135
631/1647/245/2225
631/1647/328/1649
631/1647/328/1978
639/301/930/2735
Electron microscopes
Electron microscopy
Humanities and Social Sciences
multidisciplinary
Scanning electron microscopy
Science
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Abstract Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.
AbstractList Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.
ArticleNumber 7033
Author Benedetti, Lorena
Francolini, Maura
Rodighiero, Simona
Sogne, Elisa
Milani, Paolo
Marchesi, Davide
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  givenname: Maura
  surname: Francolini
  fullname: Francolini, Maura
  organization: Fondazione Filarete for Biosciences and Innovation, Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano and National Research Council (CNR) Neuroscience Institute
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25391455$$D View this record in MEDLINE/PubMed
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Snippet Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in...
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Electron microscopes
Electron microscopy
Humanities and Social Sciences
multidisciplinary
Scanning electron microscopy
Science
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Title Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
URI https://link.springer.com/article/10.1038/srep07033
https://www.ncbi.nlm.nih.gov/pubmed/25391455
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Volume 4
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