Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 4; no. 1; p. 7033
Main Authors Benedetti, Lorena, Sogne, Elisa, Rodighiero, Simona, Marchesi, Davide, Milani, Paolo, Francolini, Maura
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 13.11.2014
Nature Publishing Group
Subjects
13
14/19
14/35
14/63
147/135
631/1647/245/2225
631/1647/328/1649
631/1647/328/1978
639/301/930/2735
Electron microscopes
Electron microscopy
Humanities and Social Sciences
multidisciplinary
Scanning electron microscopy
Science
Online AccessGet full text

Cover

More Information
Summary:Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors contributed equally to this work.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep07033