Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

• Published in: Biomedical reports Vol. 4; no. 1; pp. 97 - 101
• Main Authors: LEE, SUNG-IL, YEO, SEONG-IL, KIM, BO-BAE, KO, YOUNGKYUNG, PARK, JUN-BEOM
• Format: Journal Article
• Language: English
• Published: England D.A. Spandidos 01.01.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: cell culture techniques; Cell size; cellular spheroids; dimethylpolysiloxanes; Gingiva; Lymphocytes B; Mesenchyme; Microspheres; Morphology; Polydimethylsiloxane; Spheroids; Stem cells; Tissue engineering; United States > US; stem cells; cellular spheroids; cell culture techniques; dimethylpolysiloxanes
• ISSN: 2049-9434; 2049-9442
• DOI: 10.3892/br.2015.539

Human mesenchymal stem cells have previously been isolated and characterized from the gingiva, and gingiva-derived stem cells have been applied for tissue engineering purposes. The present study was performed to generate size-controllable stem cell spheroids using concave microwells. Gingiva-derived stem cells were isolated, and the stem cells of 1×105 (group A) or 2×105 (group B) cells were seeded in polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres was viewed under an inverted microscope, and the changes in the diameter and cell viability were analyzed. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A compared to group B. No significant changes in shape or diameter were noted with increases in incubation time. Cell viability was higher in group B at each time point when compared with group A. Within the limits of the study, the size-controllable stem cell spheroids could be generated from gingival cells using microwells. The shape of the spheroids and their viability were clearly maintained during the experimental periods.