Submillimetre Network Formation by Light-induced Hybridization of Zeptomole-level DNA

• Published in: Scientific reports Vol. 6; no. 1; p. 37768
• Main Authors: Iida, Takuya, Nishimura, Yushi, Tamura, Mamoru, Nishida, Keisuke, Ito, Syoji, Tokonami, Shiho
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.12.2016; Nature Publishing Group
• Subjects: 101/62; 631/1647/2204/2112; 639/624/1111/1117; 639/624/1111/55; Complementary DNA; Convection; Deoxyribonucleic acid; DNA; Humanities and Social Sciences; Hybridization; Light effects; multidisciplinary; Nanoparticles; Nucleotide sequence; Science; Spectroscopy
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep37768

Macroscopic unique self-assembled structures are produced via double-stranded DNA formation (hybridization) as a specific binding essential in biological systems. However, a large amount of complementary DNA molecules are usually required to form an optically observable structure via natural hybridization, and the detection of small amounts of DNA less than femtomole requires complex and time-consuming procedures. Here, we demonstrate the laser-induced acceleration of hybridization between zeptomole-level DNA and DNA-modified nanoparticles (NPs), resulting in the assembly of a submillimetre network-like structure at the desired position with a dramatic spectral modulation within several minutes. The gradual enhancement of light-induced force and convection facilitated the two-dimensional network growth near the air-liquid interface with optical and fluidic symmetry breakdown. The simultaneous microscope observation and local spectroscopy revealed that the assembling process and spectral change are sensitive to the DNA sequence. Our findings establish innovative guiding principles for facile bottom-up production via various biomolecular recognition events.