Analysis of Chlorpyrifos in Water, Fruit Juice, and Honeybee Extract by Chemiluminescent Elisa

The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection-based immobilized antigen (indirect assay) for rapid and accurate determination of chlorpyrifos in various food matrices was tested. The limit of detection (LOD) values were 1-1.75 ng mL −1 , t...

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Published inAnalytical letters Vol. 41; no. 14; pp. 2539 - 2553
Main Authors Soler, Carla, Girotti, Stefano, Ghini, Severino, Fini, Fabiana, Montoya, Angel, Manclús, Juan J., Mañes, Jordi
Format Journal Article
LanguageEnglish
Published Philadelphia, PA Taylor & Francis Group 01.01.2008
Taylor & Francis
Subjects
Analytical chemistry
Antigens
Assaying
Chemical and thermal methods
Chemiluminescence
Chemistry
Chlorpyrifos
ELISA
Exact sciences and technology
food/fruit juices
Foods
honeybee
Mathematical analysis
Miscellaneous
Chlorpyrifos
Water
Chemical analysis
Enzyme
food/fruit juices
Pesticides
Immobilization
Chemiluminescence
Foodstuff
Enzyme immunoassay
Fruit juice
honeybee
Herbicide
Elisa
Sample preparation
Detection limit
Standard curve
ELISA assay
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Summary:The suitability of competitive enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection-based immobilized antigen (indirect assay) for rapid and accurate determination of chlorpyrifos in various food matrices was tested. The limit of detection (LOD) values were 1-1.75 ng mL −1 , the standard curve midpoint (IC 50 ) was 3.5 ng mL −1 , and the assay duration was 1.5 h. Assay application to the analysis of honeybee extract resulted in chlorpyrifos recoveries varying between 62 and 83% in 5-15 ng mL −1 herbicide concentration range.
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ISSN:0003-2719
1532-236X
DOI:10.1080/00032710802363115