N-acylhomoserine lactonase-based hybrid nanoflowers: a novel and practical strategy to control plant bacterial diseases

• Published in: Journal of nanobiotechnology Vol. 20; no. 1; pp. 1 - 347
• Main Authors: Chen, Yan, Liu, Pengfu, Wu, Jiequn, Yan, Wanqing, Xie, Saixue, Sun, Xuanrong, Ye, Bang-Ce, Chu, Xiaohe
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 26.07.2022; BioMed Central; BMC
• Subjects: Adaptability; Agricultural production; AHL lactonases; Bacteria; Bacterial diseases; Bacterial diseases of plants; Bacterial infections; Biocontrol; Biological control; Burkholderia glumae; Chemical compounds; Community supported agriculture; Environmental impact; Enzyme-based hybrid nanoflower; Enzymes; Erwinia carotovora; Experiments; Fourier transforms; Health aspects; Infections; Lactones; Metalloenzymes; Morphology; Pharmacology; Plant diseases; Prevention; Proteins; Quorum sensing and quenching; Reagents; Room temperature; Scanning electron microscopy; Spectrum analysis; Stability; Synthesis; China
• ISSN: 1477-3155; 1477-3155
• DOI: 10.1186/s12951-022-01557-9

The disease caused by plant pathogenic bacteria in the production, transportation, and storage of many crops has brought huge losses to agricultural production. N-acylhomoserine lactonases (AHLases) can quench quorum-sensing (QS) by hydrolyzing acylhomoserine lactones (AHLs), which makes them the promising candidates for controlling infections of QS-dependent pathogenic bacteria. Although many AHLases have been isolated and considered as a potentially effective preventive and therapeutic agents for bacterial diseases, the intrinsically poor ambient stability has seriously restricted its application. Herein, we showed that a spheroid enzyme-based hybrid nanoflower (EHNF), AhlX@Ni.sub.3(PO.sub.4).sub.2, can be easily synthesized, and it exhibited 10 times AHL (3OC8-HSL) degradation activity than that with free AhlX (a thermostable AHL lactonase). In addition, it showed intriguing stability even at the working concentration, and retained ~ 100% activity after incubation at room temperature (25 [degrees]C) for 40 days and approximately 80% activity after incubation at 60 [degrees]C for 48 h. Furthermore, it exhibited better organic solvent tolerance and long-term stability in a complicated ecological environment than that of AhlX. To reduce the cost and streamline production processes, CSA@Ni.sub.3(PO.sub.4).sub.2, which was assembled from the crude supernatants of AhlX and Ni.sub.3(PO.sub.4).sub.2, was synthesized. Both AhlX@Ni.sub.3(PO.sub.4).sub.2 and CSA@Ni.sub.3(PO.sub.4).sub.2 efficiently attenuated pathogenic bacterial infection. In this study, we have developed N-acylhomoserine lactonase-based hybrid nanoflowers as a novel and efficient biocontrol reagent with significant control effect, outstanding environmental adaptability and tolerance. It was expected to overcome the bottlenecks of poor stability and limited environmental tolerance that have existed for over two decades and pioneered the practical application of EHNFs in the field of biological control.