Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov

• Published in: The journal of microbiology Vol. 60; no. 2; pp. 137 - 146
• Main Authors: Zhu, Wentao, Zhou, Juan, Lu, Shan, Yang, Jing, Lai, Xin-He, Jin, Dong, Pu, Ji, Huang, Yuyuan, Liu, Liyun, Li, Zhenjun, Xu, Jianguo
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.02.2022; Springer Nature B.V; 한국미생물학회
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Cardiolipin; Cardiolipins - analysis; DNA, Bacterial; Gene sequencing; Life Sciences; Lipids; Marmota - microbiology; Methylobacteriaceae - cytology; Methylobacteriaceae - genetics; Methylobacteriaceae - isolation & purification; Microbial Systematics and Evolutionary Microbiology; Microbiology; New species; Nucleotides; Phosphatidylethanolamine; Phosphatidylethanolamines - analysis; Phylogenetics; Phylogeny; Quinones; Respiratory tract; RNA, Ribosomal, 16S; rRNA 16S; Sequence Analysis, DNA; Strains (organisms); Ticks - microbiology; 생물학; Qinghai-Tibet plateau; marmot; tick; Roseomonas marmotae; Roseomonas haemaphysalidis
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-022-1428-1

Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546 T and 573) and respiratory tracts of marmots (strains 1318 T and 1311). The 16S rRNA gene sequencing revealed that strains 546 T and 573 were 97.8% identical to Roseomonas wenyumeiae Z23 T , whereas strains 1311 and 1318 T were 98.3% identical to Roseomonas ludipueritiae DSM 14915 T . In addition, a 98.0% identity was observed between strains 546 T and 1318 T . Phylogenetic and phylogenomic analyses revealed that strains 546 T and 573 clustered with R. wenyumeiae Z23 T , whereas strains 1311 and 1318 T grouped with R. ludipueritiae DSM 14915 T . The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546 T and 1318 T was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas , for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546 T (= GDMCC 1.1780 T = JCM 34187 T ) and 1318 T (= GDMCC 1.1781 T = JCM 34188 T ) as type strains, respectively.