Bacterial community evolutions driven by organic matter and powder activated carbon in simultaneous anammox and denitrification (SAD) process

• Published in: Bioresource technology Vol. 251; pp. 13 - 21
• Main Authors: Ge, Cheng-Hao, Sun, Na, Kang, Qi, Ren, Long-Fei, Ahmad, Hafiz Adeel, Ni, Shou-Qing, Wang, Zhibin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2018
• Subjects: anaerobic ammonium oxidation; Bacteria; bacterial communities; Bioreactors; carbon; Charcoal; Denitrification; denitrifying microorganisms; High throughput sequencing; Nitrogen; Organic matter; Oxidation-Reduction; PICRUSt; Planctomycetes; Power activated carbon; Proteobacteria; Simultaneous anammox and denitrification; Organic matter; High throughput sequencing; Simultaneous anammox and denitrification; PICRUSt; Power activated carbon
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2017.12.017

•A distinct shift of bacterial community driven by OM and PAC was discovered.•PAC addition mitigated the membrane fouling and stimulated denitrifiers enrichment.•PAC motivated the predominant phylum changing from Planctomycetes to Proteobacteria.•The PICRUSTs revealed an enhanced full denitrification pathway driven by OM and PAC.•Microniches such as small PAC-core particles might exist and benefit denitrifiers. A distinct shift of bacterial community driven by organic matter (OM) and powder activated carbon (PAC) was discovered in the simultaneous anammox and denitrification (SAD) process which was operated in an anti-fouling submerged anaerobic membrane bio-reactor. Based on anammox performance, optimal OM dose (50 mg/L) was advised to start up SAD process successfully. The results of qPCR and high throughput sequencing analysis indicated that OM played a key role in microbial community evolutions, impelling denitrifiers to challenge anammox’s dominance. The addition of PAC not only mitigated the membrane fouling, but also stimulated the enrichment of denitrifiers, accounting for the predominant phylum changing from Planctomycetes to Proteobacteria in SAD process. Functional genes forecasts based on KEGG database and COG database showed that the expressions of full denitrification functional genes were highly promoted in RC, which demonstrated the enhanced full denitrification pathway driven by OM and PAC under low COD/N value (0.11).