Genomic characterization of echovirus 6 causing aseptic meningitis in Hokkaido, Japan: a novel cluster in the nonstructural protein coding region of human enterovirus B

• Published in: Archives of virology Vol. 158; no. 4; pp. 775 - 784
• Main Authors: Miyoshi, Masahiro, Komagome, Rika, Ishida, Setsuko, Nagano, Hideki, Takahashi, Kenichi, Okano, Motohiko
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.04.2013; Springer Nature B.V
• Subjects: Amino Acid Sequence; Australia; Base Sequence; Biomedical and Life Sciences; Biomedicine; chemistry; Child, Preschool; China; classification; coat proteins; Conserved Sequence; disease outbreaks; DNA-directed RNA polymerase; Echovirus; Echovirus 6, Human; Echovirus 6, Human - classification; Echovirus 6, Human - genetics; Echovirus 6, Human - isolation & purification; Echovirus Infections; Echovirus Infections - epidemiology; Echovirus Infections - virology; Enterovirus; Enterovirus B; epidemiology; Genes; genetics; Genome, Viral; Genomes; Humans; Infectious Diseases; isolation & purification; Japan; Japan - epidemiology; Medical Microbiology; Meningitis; Meningitis, Aseptic; Meningitis, Aseptic - epidemiology; Meningitis, Aseptic - virology; Meningitis, Viral; Meningitis, Viral - epidemiology; Meningitis, Viral - virology; Molecular Sequence Data; nucleotide sequences; Original Article; patients; Phylogenetics; Phylogeny; proteinases; Proteins; RNA polymerase; RNA, Viral; RNA, Viral - chemistry; RNA, Viral - isolation & purification; South Korea; Viral Nonstructural Proteins; Viral Nonstructural Proteins - genetics; Virology; Japan; United States > US; China; South Korea; Australia; Complete Nucleotide Sequence; Nonstructural Protein; Aseptic Meningitis; Nucleotide Sequence Identity; Acute Flaccid Paralysis
• ISSN: 0304-8608; 1432-8798; 1432-8798
• DOI: 10.1007/s00705-012-1535-0

We determined four complete nucleotide sequences of echovirus 6 (E6) isolated from an epidemic of aseptic meningitis (AM) in Hokkaido, Japan, in 2011. Phylogenetic analysis of the genes encoding viral capsid protein 1 revealed that the strains were closely related to E6 strains isolated in China in recent years, but they were distantly related to E6 strains isolated from patients with AM in Osaka Prefecture, Japan, in 2011. The genes encoding the viral protease and RNA-dependent RNA polymerase (3CD) were closely related to those of several non-E6 strains of the species Human enterovirus B isolated in China, South Korea, and Australia from 1999 to 2010, resulting in a novel cluster in the phylogenetic tree. These results suggest that the incidence of AM in Japan in 2011 was caused by at least two lineages of E6 strains, and a lineage of the 3CD gene was interspersed among different serotypic strains isolated in Western Pacific countries.