A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics

Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transpo...

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Published inScientific reports Vol. 3; no. 1; p. 2052
Main Authors Wend, Sabrina, Bosco, Cristina Dal, Kämpf, Michael M., Ren, Fugang, Palme, Klaus, Weber, Wilfried, Dovzhenko, Alexander, Zurbriggen, Matias D.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 21.06.2013
Nature Publishing Group
Subjects
631/1647
631/449/1741/1576
631/449/2675
631/61/447/2311
Biosensing Techniques
Biosensors
Cell Line
Humanities and Social Sciences
Humans
Indoleacetic Acids - analysis
Kinetics
Limit of Detection
multidisciplinary
Plant cells
Plants - chemistry
Science
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Summary:Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.
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content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/srep02052