MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2)

• Published in: Journal of applied oral science Vol. 23; no. 3; pp. 265 - 271
• Main Authors: Ponce-Bravo, Santa, Ledesma-Montes, Constantino, Garcés-Ortíz, Maricela
• Format: Journal Article
• Language: English
• Published: Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01.06.2015; Faculdade De Odontologia De Bauru - USP; University of São Paulo
• Subjects: Adenoma, Pleomorphic - chemistry; Adenoma, Pleomorphic - pathology; Antibodies, Monoclonal; Biomarkers, Tumor - analysis; Case-Control Studies; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Glycolipids; Glycoproteins; Human milk fat globule membrane; Humans; Immunohistochemistry; Membrane Proteins; MUC-1 mucin; Mucin-1 - analysis; Myoepithelial cell; Original; Paraffin Embedding; Pleomorphic adenoma; Reference Values; Salivary Gland Neoplasms - chemistry; Salivary Gland Neoplasms - pathology; Salivary gland tumors; Salivary Glands - chemistry; Salivary Glands - secretion; Staining and Labeling - methods; Human milk fat globule membrane; Myoepithelial cell; MUC-1 mucin; Salivary gland tumors; Pleomorphic adenoma
• ISSN: 1678-7757; 1678-7765; 1678-7765; 1678-7757
• DOI: 10.1590/1678-775720140481

Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors. The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue. Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used. Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive. Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.