Thyroxine-dependent modulation of actin polymerization in cultured astrocytes. A novel, extranuclear action of thyroid hormone

• Published in: The Journal of biological chemistry Vol. 265; no. 9; pp. 5296 - 5302
• Main Authors: SIEGRIST-KAISER, C. A, JUGE-AUBRY, C, TRANTER, M. P, EKENBARGER, D.K M, LEONARD, J. L
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25.03.1990
• Subjects: Actins - metabolism; Animals; astrocytes; Astrocytes - drug effects; Astrocytes - metabolism; Biological and medical sciences; Bucladesine - pharmacology; Cell Nucleus - metabolism; Cell physiology; Cells, Cultured; Cycloheximide - pharmacology; Cytoskeleton - metabolism; Cytoskeleton - ultrastructure; Dactinomycin - pharmacology; Fundamental and applied biological sciences. Psychology; Hormonal regulation; Iodide Peroxidase - antagonists & inhibitors; Kinetics; Macromolecular Substances; Molecular and cellular biology; thyronine; Thyroxine - pharmacology; Cell culture; Rat; Gel electrophoresis; Rodentia; Actins; Microfilament; Astrocyte; Iodothyronine deiodase-5; Inactivation; Depolymerization; Vertebrata; Regulation(control); Mammalia; Messenger RNA; Blotting assay; Hormonal regulation; Cytoskeleton; Thyroid hormone; Kinetics
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/s0021-9258(19)34121-3

Actin depolymerization specifically blocks the rapid thyroid hormone-dependent inactivation of type II iodothyronine 5'-deiodinase. Thyroid hormone appears to regulate enzyme inactivation by modulating actin-mediated internalization of this plasma membrane-bound protein. In this study, we examined the interrelationships between thyroxine-dependent enzyme inactivation and the organization of the actin cytoskeleton in cultured astrocytes. Steady-state enzyme levels were inversely related to actin content in dibutyryl cAMP-stimulated astrocytes, and increases in filamentous actin resulted in progressively shorter enzyme half-lives without affecting enzyme synthesis. In the absence of thyroxine, filamentous actin decreased by approximately 40% and soluble actin correspondingly increased; thyroxine normalized filamentous actin levels without changing total cell actin. Thyroxine treatment for only 10 min resulted in an approximately 50% loss of enzyme and increased filamentous actin 2-fold. Neither cycloheximide nor actinomycin D affected the thyroxine-induced actin polymerization. Astrocytes grown without thyroxine also showed a disorganized actin cytoskeleton, and 10 nM thyroxine or 10 nM reverse triiodothyronine normalized the actin cytoskeleton appearance within 20 min; 10 nM 3,3',5-triiodothyronine had no effect. These data show that thyroxine modulates the organization of the actin cytoskeleton in astrocytes and suggest that regulation of actin polymerization may contribute to thyroid hormone's influence on arborization, axonal transport, and cell-cell contact in the developing brain.