Nucleic acid probe containing fluorescent tricyclic base-linked acyclonucleoside for detection of single nucleotide polymorphisms

The development of a reliable and simple method for detecting single nucleotide polymorphisms (SNPs), common genetic variations in the human genome, is currently an important research area because SNPs are important for identifying disease-causing genes and for pharmacogenetic studies. Here, we deve...

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Published inBioorganic & medicinal chemistry Vol. 20; no. 1; pp. 16 - 24
Main Authors Furukawa, Kinji, Hattori, Mayumi, Ohki, Tokimitsu, Kitamura, Yoshiaki, Kitade, Yukio, Ueno, Yoshihito
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier Ltd 01.01.2012
Elsevier
Subjects
Acyclonucleoside
Biological and medical sciences
chemistry
Detection
DNA
DNA - chemistry
DNA - metabolism
DNA probes
DNA Probes - chemical synthesis
DNA Probes - chemistry
fluorescence
Fluorescent Dyes - chemistry
genes
genetic variation
Genome, Human
Humans
Imidazoles - chemical synthesis
Imidazoles - chemistry
Medical sciences
methodology
Nucleic Acid Denaturation
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - chemistry
Pharmacology. Drug treatments
Polymorphism, Single Nucleotide
Pyrimidines - chemical synthesis
Pyrimidines - chemistry
RNA
RNA - chemistry
RNA - metabolism
single nucleotide polymorphism
SNPs
Spectrometry, Fluorescence
Transition Temperature
Tricyclic base
SNPs
Acyclonucleoside
Detection
Tricyclic base
DNA
Acyclic nucleoside
Genotype
Single nucleotide polymorphism
Fluorescent compound
Tricyclic compound
Nucleic acid
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Summary:The development of a reliable and simple method for detecting single nucleotide polymorphisms (SNPs), common genetic variations in the human genome, is currently an important research area because SNPs are important for identifying disease-causing genes and for pharmacogenetic studies. Here, we developed a novel method for SNP detection. We designed and synthesized DNA probes containing a fluorescent tricyclic base-linked acyclonucleoside P. The type of nucleobases involved in the SNP sites in the DNA and RNA targets could be determined using four DNA probes containing P. Thus, this system would provide a novel and simple method for detecting SNPs in DNA and RNA targets.
Bibliography:http://dx.doi.org/10.1016/j.bmc.2011.11.045
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ISSN:0968-0896
1464-3391
DOI:10.1016/j.bmc.2011.11.045