Cloning, characterization, and functional expression of cDNAs encoding glucose transporter proteins from the human parasite Schistosoma mansoni
The blood-dwelling adult form of the parasitic worm, Schistosoma mansoni, consumes prodigious quantities of host glucose following its transport across the tegument. Immunologic or pharmacologic targeting of the relevant transporter proteins might form the basis of a schistosome control strategy. He...
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Published in | The Journal of biological chemistry Vol. 269; no. 6; pp. 4247 - 4253 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Biochemistry and Molecular Biology
11.02.1994
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Subjects | |
Online Access | Get full text |
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Summary: | The blood-dwelling adult form of the parasitic worm, Schistosoma mansoni, consumes prodigious quantities of host glucose following
its transport across the tegument. Immunologic or pharmacologic targeting of the relevant transporter proteins might form
the basis of a schistosome control strategy. Here we report the isolation and characterization of three different full-length
cDNAs whose predicted protein sequences show a high degree of sequence and structural similarity to the facilitated diffusion
transporter proteins of other animals, plants, and bacteria. Functional expression of two cDNAs has been achieved by injection
of Xenopus oocytes with in vitro derived sense strand RNA. Injected oocytes have a significantly increased ability to take
up radiolabeled glucose analogues over controls. S. mansoni glucose transporters expressed in oocytes exhibit stereospecificity
for D-glucose, have relaxed specificity for different hexoses, exhibit sodium independence, and are markedly inhibited by
phloretin and cytochalasin B. These two transporters, expressed in oocytes, have a Km for 3-O-methylglucose of 1.3 and 2 mM.
A third glucose transporter homologue cDNA appears to derive from a recent pseudogene. Both of the functional S. mansoni glucose
transporter genes are expressed in larval and adult male and female schistosomes. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/s0021-9258(17)41770-4 |